Rat CXCL1 ELISA Kit KE20033 proteintech
$299.00
In stock
SKU
KE20033
| Product name | Rat CXCL1 ELISA Kit |
| Synonyms | Cinc1,CINC-1,C-X-C motif chemokine 1,Cxcl1,Cytokin |
| Reactivity | Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 3.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81503 |
| Sample Type | Average Range |
| Rat serum | 77% 70%-83% |
| Cell culture supernatant | 91% 89%-96% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 991.4 85.3 8.6 |
| 2 | 16 235.4 16.0 6.8 |
| 3 | 16 113.3 10.0 8.8 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CXC motif chemokine ligand 1 (CXCL1), a CXC chemokine, is also known as growth-regulated (or-related) oncogene-α (Gro-α) . Its most important property is to cause chemotaxis of immune cells, mainly neutrophils, and to a lesser extent CD14+ monocytes and basophils , but not T cells. The main receptor for CXCL1 is CXCR2, activated at concentrations of just a few nanomoles of CXCL1. Any inflammatory response is associated with an increase in the level of pro-inflammatory cytokines which induce an increase in CXCL1 expression. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Cinc1,CINC-1,C-X-C motif chemokine 1,Cxcl1,Cytokin FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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