| Product name | Human FOXF1 ELISA Kit |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.313-20 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2294 |
| Sample Type | Average Range |
| Cell lysate | 115% 110%-121% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 11.38 0.51 4.49 |
| 2 | 16 2.88 0.15 5.18 |
| 3 | 16 0.95 0.05 5.10 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
FOXF1 (Forkhead box protein F1), also known as FKHL5, It is expected to be located in the nucleus, which is expressed predominantly in lung and placenta. FOXF1, a member of the Forkhead box family of transcription factors, as one of the most highly and consistently upregulated genes in FP-RMS. While it is known that FOXF1 is required for mesodermal differentiation toward endothelial and smooth muscle cell lineages, FOXF1 is not expressed in differentiated skeletal muscle cells, and its role in fusion-positive FP-RMS remains unknown. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.