Human HE4/WFDC2 ELISA Kit KE00265 proteintech
$299.00
In stock
SKU
KE00265
| Product name | Human HE4/WFDC2 ELISA Kit |
| Synonyms | WFDC2, HE4, dJ461P17.6, WAP5 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Saliva，Urine |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 46.88-3000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10406 |
| Sample Type | Average Range |
| Human plasma | 99% 80%-120% |
| Urine | 99% 80%-128% |
| Saliva | 99% 77%-106% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 2,776.2 150.1 5.4 |
| 2 | 24 685.3 22.5 3.3 |
| 3 | 24 172.6 16.8 9.7 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Human epididymis protein 4 (HE4), also known as WFDC2, was originally identified as a transcript exclusively expressed in the epididymis and was proposed as a specific marker for this tissue. HE4 is a member of the Whey Acidic Protein (WAP) domain family of proteins. HE4 is expressed in a number of normal human tissues outside of the male reproductive system, including the trachea, lung and nasal epithelium and is also found in a subset of pulmonary epithelial-derived tumour cell lines. At present, HE4 serves as a serum biomarker of ovarian cancer, and has better sensitivity and specificity than CA125. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms WFDC2, HE4, dJ461P17.6, WAP5 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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