Human HNF4A ELISA Kit KE00634 proteintech
$299.00
In stock
SKU
KE00634
| Product name | Human HNF4A ELISA Kit |
| Synonyms | Hepatocyte nuclear factor 4-alpha, HNF 4 alpha, HNF4, HNF4a7, HNF4a8 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.625-40 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3172 |
| Sample Type | Average Range |
| Cell lysate | 81% 78%-83% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 20.07 1.88 9.35 |
| 2 | 16 4.84 0.37 7.65 |
| 3 | 16 2.46 0.15 5.91 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals. Moreover, reduced expression of HNF4A has been reported in liver fibrosis, and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for treating liver fibrosis. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Hepatocyte nuclear factor 4-alpha, HNF 4 alpha, HNF4, HNF4a7, HNF4a8 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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