| Product name | Rat IFN-gamma ELISA Kit |
| Synonyms | IFN Gamma,Ifng,IFN-gamma,interferon gamma,IFN γ |
| Reactivity | Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 9.5 pg/mL |
| Range | 62.5-4000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 25712 |
| Sample Type | Average Range |
| Rat serum | 102% 86%-124% |
| Cell culture supernatant | 97% 75%-129% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 1,744.7 153.6 8.8 |
| 2 | 24 381.4 21.4 5.6 |
| 3 | 24 93.0 7.4 7.9 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| rat | tissue homogenates J Cardiothorac Surg Resveratrol ameliorates myocardial injury and inflammatory response and cell apoptosis in Kawasaki disease rats by activating the Nrf2/HO-1 pathway. Authors - Weiyan Yan View Article |
The IFNs were originally discovered as agents that interfere with viral replication. Initially, they were classified by the secreting cell type but are now classified into type I and type II according to receptor specificity and sequence homology. Interferon gamma (Ifng) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant Ifng expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms IFN Gamma,Ifng,IFN-gamma,interferon gamma,IFN γ FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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