Human MAFF ELISA Kit KE00953 proteintech
$299.00
In stock
SKU
KE00953
| Product name | Human MAFF ELISA Kit |
| Synonyms | U MAF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23764 |
| Sample Type | Average Range |
| Cell lysate | 90% 83%-95% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 5.20 0.51 9.72 |
| 2 | 16 1.26 0.15 12.27 |
| 3 | 16 0.61 0.06 9.26 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
MAFF is a member of the small Maf protein family (MafF, MafG, MafK) within the basic region leucine zipper (bZIP) transcription factor superfamily. It can form homodimers or heterodimers with other bZIP proteins (e.g., Nrf2, Bach1) to regulate gene expression by binding to antioxidant response elements (AREs) or Maf recognition elements (MAREs). MAFF plays critical roles in cellular defense against oxidative stress, erythrocyte differentiation, and neuronal development. It is highly expressed in specific tissues, including the liver, kidney, and hematopoietic cells. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms U MAF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Reviews
Write Your Own Review