Human Methylmalonyl Coenzyme A mutase ELISA Kit KE00830 proteintech
$299.00
In stock
SKU
KE00830
| Product name | Human Methylmalonyl Coenzyme A mutase ELISA Kit |
| Synonyms | MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl-CoA isomerase |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4594 |
| Sample Type | Average Range |
| Cell lysate | 98% 98%-99% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 5.34 0.42 7.85 |
| 2 | 16 1.32 0.05 3.96 |
| 3 | 16 0.72 0.04 6.09 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA, resulting in life-threatening metabolic acidosis, respiratory distress, neurological impairment, hyperammonemia, and ketosis. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl-CoA isomerase FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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