| Product name | Human TREM-1/CD354 ELISA Kit |
| Synonyms | TREM1, CD354, TREM 1 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Saliva, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 7.8-500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54210 |
| Sample Type | Average Range |
| Human serum | 79% 75%-86% |
| Cell culture supernatant | 104% 101%-107% |
| Saliva | 106% 101%-110% |
| Cell lysate | 103% 77%-122% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 253.6 14.0 5.5 |
| 2 | 16 60.7 2.9 4.8 |
| 3 | 16 31.0 2.0 6.4 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Triggering receptor expressed on myeloid cells-1 (TREM-1, also known as CD354) is a member of the immunoglobulin superfamily. It plays a crucial role in the innate immune response to bacterial and fungal infections by triggering the release of pro-inflammatory chemokines and cytokines and increasing the surface expression of cell activation markers. TREM-1 also exists as a soluble receptor (sTREM-1) that arises from proteolytic cleavage of the membrane-bound receptor by matrix metalloproteases. The serum levels of sTREM-1 may correlate with disease severity and treatment outcomes in pulmonary tuberculosis patients. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms TREM1, CD354, TREM 1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.