Human NSD3 ELISA Kit KE00541 proteintech
$299.00
In stock
SKU
KE00541
| Product name | Human NSD3 ELISA Kit |
| Synonyms | NSD3, WHSC1L1, DC28, EC:2.1.1.370, Histone-lysine N-methyltransferase NSD3 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 19.5-1250 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54904 |
| Sample Type | Average Range |
| Cell lysate | 101% 95%-111% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 609.5 45.9 7.5 |
| 2 | 16 149.4 8.5 5.7 |
| 3 | 16 73.0 6.2 8.5 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
NSD3, also named as WHSC1L1 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms NSD3, WHSC1L1, DC28, EC:2.1.1.370, Histone-lysine N-methyltransferase NSD3 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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