| Product name | Human NUP214 ELISA Kit |
| Synonyms | CAIN, CAN, KIAA0023 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.48 ng/mL |
| Range | 1.56-100 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8021 |
| Sample Type | Average Range |
| Cell lysate | 82% 75%-91% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 47.05 1.78 3.78 |
| 2 | 16 12.49 0.44 3.51 |
| 3 | 16 6.60 0.18 2.70 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
NUP214, also known as CAN, is a nucleoporin that is an essential component of the nuclear pore complex (NPC), which plays a crucial role in nucleocytoplasmic transport. It is a large protein with a molecular weight of approximately 214 kDa and contains multiple domains that facilitate its interaction with other proteins and nucleic acids. It is often involved in fusion genes in leukemia, such as SET-NUP214, which is associated with poor prognosis in T-cell acute lymphoblastic leukemia. NUP214 rearrangements alone are insufficient to induce leukemia but contribute to drug resistance. NUP214 also interacts with other nucleoporins and is essential for maintaining normal cellular functions. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CAIN, CAN, KIAA0023 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.