Human PARP2 ELISA Kit KE00544 proteintech
$299.00
In stock
SKU
KE00544
| Product name | Human PARP2 ELISA Kit |
| Synonyms | PARP2, ADPRT2, ADPRTL3, ARTD2, DNA ADP-ribosyltransferase PARP2 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 3.5 pg/mL |
| Range | 39-2500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10038 |
| Sample Type | Average Range |
| Cell lysate | 83% 81%-87% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 1,645.8 189.5 11.5 |
| 2 | 16 296.3 31.9 10.8 |
| 3 | 16 145.8 16.0 11.0 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. PARP2 protect from illegitimate recombinations in the context of high genomic instability, which contains an N-terminal regulatory domain and a C-terminal enzymatic domain. PARP2 itself gets activated by PAR and plays an important role in the branched PAR chain polymerization. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms PARP2, ADPRT2, ADPRTL3, ARTD2, DNA ADP-ribosyltransferase PARP2 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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