Rat VEGF ELISA Kit KE20027 proteintech
$299.00
In stock
SKU
KE20027
| Product name | Rat VEGF ELISA Kit |
| Synonyms | Vegfa, VEGF164 |
| Reactivity | Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 83785 |
| Sample Type | Average Range |
| Rat serum | 81% 72%-100% |
| Cell culture supernatant | 89% 85%-99% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 1,155.7 101.0 8.7 |
| 2 | 24 330.2 17.9 5.4 |
| 3 | 24 297.2 22.0 7.4 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| rat | cell lysates Biomaterials Topology scaffolds-enhanced paracrine of BMSCs through mechanotransduction-related metabolism reprogramming for burn wounds healing Authors - Qingrong Zhang View Article |
Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Vegfa, VEGF164 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
- Bone transport accelerates diabetic foot ulcer healing via TGF-β1-mediated angiogenic and osteo-immune coupling.
Journal: J Mol Histol | Authors: Yan Chen | Species: rat | Application: Serum - Topology scaffolds-enhanced paracrine of BMSCs through mechanotransduction-related metabolism reprogramming for burn wounds healing
Journal: Biomaterials | Authors: Qingrong Zhang | Species: rat | Application: cell lysates
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