Human SOCS3 ELISA Kit KE00879 proteintech
$299.00
In stock
SKU
KE00879
| Product name | Human SOCS3 ELISA Kit |
| Synonyms | ATOD4, CIS3, CIS-3, Cytokine-inducible SH2 protein 3, SOCS-3 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.10 ng/mL |
| Range | 0.625-40 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9021 |
| Sample Type | Average Range |
| Cell lysate | 76% 73%-79% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 20.86 1.36 6.50 |
| 2 | 16 5.14 0.19 3.70 |
| 3 | 16 1.57 0.10 6.66 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
The suppressor of cytokine signaling (SOCS) family proteins are negative feedback system that inhibits cytokine signal transduction. One of the SOCS family, SOCS3, also named CIS3 and SSI3, is involved in inhibition of JAK/STAT pathway which regulates IL-6 signaling in vivo. SOCS3 can be rapidly trigger by a number of factors including interleukins, IFN-gamma and TNF-alpha. Pathology such as inflammatory response, wound repair and obesity and so on have been linked to SOCS3. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms ATOD4, CIS3, CIS-3, Cytokine-inducible SH2 protein 3, SOCS-3 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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