Human TBX21/T-bet ELISA Kit KE00524 proteintech
$299.00
In stock
SKU
KE00524
| Product name | Human TBX21/T-bet ELISA Kit |
| Synonyms | TBX21, TBX21,T-bet, T bet, T-box protein 21, T-box transcription factor TBX21 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 9.1 pg/mL |
| Range | 39.1-2500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 30009 |
| Sample Type | Average Range |
| Cell lysate | 83% 70%-94% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 1,182.7 31.2 2.6 |
| 2 | 16 290.8 9.7 3.3 |
| 3 | 16 149.4 4.7 3.2 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
TBX21, also known as T-bet, is a key T-box transcription factor that plays a critical role in the regulation of immune responses and the development of Th1 cells. The gene is a member of the phylogenetically conserved T-box family, which shares a common DNA-binding domain. T-bet is predominantly expressed in Th1 cells and natural killer (NK) cells, where it controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFN-γ). This transcription factor is essential for initiating Th1 lineage development from naïve T precursor cells. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms TBX21, TBX21,T-bet, T bet, T-box protein 21, T-box transcription factor TBX21 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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