Rat TNF-alpha ELISA Kit KE20018 proteintech
$299.00
In stock
SKU
KE20018
| Product name | Rat TNF-alpha ELISA Kit |
| Synonyms | TNF-α, TNF alpha, Tnfa, RATTNF, TNF-a, Tnf |
| Reactivity | Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 4.7-300 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24835 |
| Sample Type | Average Range |
| Cell culture supernatant | 98% 80%-129% |
| Rat serum | 77% 66%-91% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 137.6 10.2 7.4 |
| 2 | 24 31.3 1.3 4.1 |
| 3 | 24 8.4 0.5 6.4 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| rat | serum ESC Heart Fail Activation of AHR by ITE improves cardiac remodelling and function in rats after myocardial infarction Authors - Xiaoyan Lin View Article |
| FH Miriam (Verified Customer) (12-10-2025) | Accurate TNF quantification Applications: Other Cell Tissue Type: Brain |
| FH Isabel (Verified Customer) (10-15-2024) | A reliable kit where you do not have to use a reduced volume with good sensitivity. Applications: Other Cell Tissue Type: Rat GD15 plasma |
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Rat and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the rat form is glycosylated. In rat deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms TNF-α, TNF alpha, Tnfa, RATTNF, TNF-a, Tnf FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
- Nanoparticles loaded with a CSF1R antagonist selectively depletes microglial cells and modulates inflammation in spinal cord injury
Journal: J Control Release | Authors: Jingjing Yang | Species: rat | Application: cell culture supernatants - Immune-activated microspheres for enhanced chemoembolization of hepatocellular carcinoma by blocking the adenosine A2A receptor
Journal: Acta Biomater | Authors: Minjiang Chen | Species: rat | Application: serum - Spinster homolog 2/S1P signaling ameliorates macrophage inflammatory response to bacterial infections by balancing PGE2 production
Journal: Cell Commun Signal | Authors: Chao Fang | Species: rat | Application: cell culture supernatants - Panax ginseng alleviates diabetic peripheral neuropathy by suppressing mitochondrial dysfunction and oxidative stress via modulation of the RAGE, NF-κB, and Nrf2 pathways.
Journal: J Ginseng Res | Authors: Pengcheng Liu | Species: rat | Application: cell culture supernatants - Activation of AHR by ITE improves cardiac remodelling and function in rats after myocardial infarction
Journal: ESC Heart Fail | Authors: Xiaoyan Lin | Species: rat | Application: serum - Identification of the key role of IL-17RB in the treatment of osteoarthritis with Shaoyao Gancao decoction: Verification based on RNA-seq and bioinformatics analysis
Journal: PLoS One | Authors: Chengzhi Hou | Application: serum - Nanoparticles loaded with a CSF1R antagonist selectively depletes microglial cells and modulates inflammation in spinal cord injury
Journal: J Control Release | Authors: Jingjing Yang | Species: rat - Spinster homolog 2/S1P signaling ameliorates macrophage inflammatory response to bacterial infections by balancing PGE2 production
Journal: Cell Commun Signal | Authors: Chao Fang | Species: rat - Integrated gut microbiome and UHPLC-MS metabolomics to reveal the prevention mechanism of pidanjiangtang granules on IGT Rats
Journal: Phytomedicine | Authors: Yu Xie | Species: rat - In Situ Forming ROS-Scavenging Hydrogel with a STING Inhibitor Improves Recovery After Acute Spinal Cord Injury by Modulating Inflammatory Microenvironment.
Journal: Adv Healthc Mater | Authors: Xigong Li | Species: rat | Application: ELISA - Immune-activated microspheres for enhanced chemoembolization of hepatocellular carcinoma by blocking the adenosine A2A receptor
Journal: Acta Biomater | Authors: Minjiang Chen | Species: rat - Bioactive polymethoxylated flavones from Clerodendranthus spicatus alleviate high-fat diet-induced lipid dysfunction and metabolic inflammation via targeting the PPARα/NF-κB pathway.
Journal: Food Res Int | Authors: Zheng Li | Species: rat - KDM4C regulates trophoblast proliferation in preeclampsia via the NFATc4/Wnt pathway
Journal: Int J Biol Macromol | Authors: Changxin Shao | Species: rat - Panax ginseng alleviates diabetic peripheral neuropathy by suppressing mitochondrial dysfunction and oxidative stress via modulation of the RAGE, NF-κB, and Nrf2 pathways.
Journal: J Ginseng Res | Authors: Pengcheng Liu | Species: rat - Electroacupuncture attenuates synovitis in knee osteoarthritis and is associated with modulation of the protein S-TAM (Axl/MerTK)-Rac1 signaling axis.
Journal: Front Immunol | Authors: Meng-Meng Li | Species: rat - Exploring the dosage-effect relationship and mechanism of Huangqi in Huangqi Guizhi Wuwu decoction for treating rheumatoid arthritis based on the compatibility of the formula.
Journal: J Ethnopharmacol | Authors: Xiaohong Gong | Species: rat - Dachaihu decoction ameliorates septic intestinal injury via modulating the gut microbiota and glutathione metabolism as revealed by multi-omics
Journal: J Ethnopharmacol | Authors: Na Huang | Species: rat - Omics-based pharmacological evaluation reveals Yuanhu Zhitong oral liquid ameliorates arthritis by regulating PKC/ERK/NF-κB signaling pathway
Journal: J Ethnopharmacol | Authors: Kai Zhang | Species: rat - Dachaihu decoction ameliorates septic intestinal injury via modulating the gut microbiota and glutathione metabolism as revealed by multi-omics
Journal: J Ethnopharmacol | Authors: Na Huang | Species: rat - AD16 Modulates Microglial Activation and Polarization to Mitigate Neuroinflammation in Ischemic Stroke Models Through α7nAChR-ERK-STAT3 Signaling
Journal: CNS Neurosci Ther | Authors: Guo-Jian Zhao | Species: rat - The mechanisms by which hypothalamic neuroinflammation induced by neonatal cerebral ischemia-hypoxia leads to decreased thymic function via the HPA axis.
Journal: Cell Biosci | Authors: Gai-Gai Liu | Species: rat | Application: ELISA - Empagliflozin alleviates hepatic ischemia-reperfusion injury by inhibiting c-Myc through the JAK1-STAT3 signaling pathway
Journal: Int Immunopharmacol | Authors: Alimu Tulahong | Species: rat - Procyanidin B2 ameliorates hypoxic-ischemic brain damage in neonatal rats by inhibiting pyroptosis via the TLR4/NF-κB signaling pathway.
Journal: Int Immunopharmacol | Authors: Ting Zhang | Species: rat - pH-Responsive Carboxymethyl Cellulose-Encapsulating Hesperidin-Selenium Nanoparticles Attenuate Paracetamol-Induced Acute Kidney Injury via Keap-1/Nrf2, NF-κB, and Mitochondrial Apoptosis Modulation.
Journal: Int J Mol Sci | Authors: Mohamed H. A. Gadelmawla | Species: rat - Procyanidin B2 ameliorates hypoxic-ischemic brain damage in neonatal rats by inhibiting pyroptosis via the TLR4/NF-κB signaling pathway.
Journal: Int Immunopharmacol | Authors: Ting Zhang | Species: rat - Areca Thirteen Pill Improves Depression in Rat by Modulation of the Chemokine/Chemokine Receptor Axis
Journal: Mol Neurobiol | Authors: Jing-Jing Bai | Species: rat - Investigating the regulation of the miR-199a-3p/TGF-β/Smad signaling pathway by BSHXF drug-containing serum combined with ADSCs for delaying intervertebral disc degeneration
Journal: Front Pharmacol | Authors: Enxu Liu | Species: rat | Application: ELISA - Identification of claudin-2 as a promising biomarker for early diagnosis of pre-diabetes
Journal: Front Pharmacol | Authors: Yang Songtao - Jiawei Danxuan Koukang and Its Component Kaempferol Alleviate Oral Submucous Fibrosis by Restoring Epithelial-Fibroblast Homeostasis and Suppressing Neutrophil Recruitment via the ANXA1/FPR2 Axis.
Journal: Food Sci Nutr | Authors: Yao Xiao | Species: rat - Hyperglycemia and circadian disruption lead to retinal dysfunction in a stabilized colony of the fat sand rat Psammomys obesus
Journal: Biochim Biophys Acta Mol Basis Dis | Authors: Catherine E Estay-Ahumada | Species: rat | Application: ELISA - Pathogenesis of impaired glucose tolerance in rats based on transcriptome analysis.
Journal: Sci Rep | Authors: Ran Yi | Species: rat | Application: ELISA - Febuxostat alleviates testicular dysfunction induced by cyclophosphamide in rats via modulation of pyroptosis and mTOR autophagy axis.
Journal: Sci Rep | Authors: Hanan Abdelmawgoud Atia | Species: rat - Targeting CDK1 inhibits Golgi apparatus stress-mediated neuroinflammation and neuronal apoptosis after intracerebral hemorrhage by modulating GRASP55 phosphorylation
Journal: Cell Signal | Authors: Xiaobo Zhang | Species: rat - Blockade of the Notch Pathway by Inhibition of Fucosylation Ameliorates Chondrocyte Inflammation and Extracellular Matrix Degradation
Journal: J Inflamm Res | Authors: Yunheng Zhang | Species: rat | Application: ELISA - Transcranial magnetic stimulation through attenuating blood-spinal cord barrier disruption and reducing inflammatory response to improve motor function in rats with spinal cord injury
Journal: Brain Res Bull | Authors: Qingqin Xu | Species: rat - Stigmasterol upregulates Akt/mTOR/p70S6K signaling through SYVN1 to alleviate osteoporosis in ovariectomized rats.
Journal: Eur J Nutr | Authors: Fuping Zhu | Species: rat | Application: ELISA - Exploring the protective role of green tea extract against cardiovascular alterations induced by chronic REM sleep deprivation via modulation of inflammation and oxidative stress
Journal: BMC Complement Med Ther | Authors: Yonca Coluk | Species: rat - Sodium Selenite Ameliorates Silver Nanoparticles Induced Vascular Endothelial Cytotoxic Injury by Antioxidative Properties and Suppressing Inflammation Through Activating the Nrf2 Signaling Pathway
Journal: Biol Trace Elem Res | Authors: Yunyun Ma | Species: rat - ARIH1 Promotes Preeclampsia by Inducing MFN2-Dependent Hypoxia-Triggered Mitophagy and Endoplasmic Reticulum Stress in Trophoblasts.
Journal: FASEB J | Authors: Hongyu Liu | Species: rat | Application: ELISA - Activation of AHR by ITE improves cardiac remodelling and function in rats after myocardial infarction
Journal: ESC Heart Fail | Authors: Xiaoyan Lin | Species: rat - Incontinence-Associated Dermatitis-Like Skin Changes Induced by the Application of Absorbent Pads Containing Bacteria and Artificial Urine in Rats
Journal: Exp Dermatol | Authors: Ai Horinouchi | Species: rat - Mechanism of action of total saponin Achyranthes in treating knee osteoarthritis explored using network pharmacology and animal experimentation
Journal: Iran J Basic Med Sci | Authors: Shiwei Zhuang | Species: rat - Identification of the key role of IL-17RB in the treatment of osteoarthritis with Shaoyao Gancao decoction: Verification based on RNA-seq and bioinformatics analysis
Journal: PLoS One | Authors: Chengzhi Hou | Application: ELISA - Shen-Ling-Bai-Zhu-San alleviates ulcerative colitis by enhancing mitophagy via the Nrf2/PINK1/Parkin pathway.
Journal: J Mol Histol | Authors: Huan Wang | Species: rat | Application: ELISA - An's anorectal fumigation lotion for nuclear factor kappa-B pathway-targeted treatment of inflammatory mixed hemorrhoids.
Journal: J Tradit Chin Med | Authors: Feng Dayong | Species: rat - Resveratrol ameliorates myocardial injury and inflammatory response and cell apoptosis in Kawasaki disease rats by activating the Nrf2/HO-1 pathway.
Journal: J Cardiothorac Surg | Authors: Weiyan Yan | Species: rat | Application: ELISA - Stigmasterol, the Active Ingredient in Huanglian Decoction, Inhibits the MAOA-NF-κB-MLCK Pathway to Improve the Inflammation in Rats With Reflux Esophagitis.
Journal: Endocr Metab Immune Disord Drug Targets | Authors: Huan Wang | Species: rat
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