Human TIGAR ELISA Kit KE00989 proteintech
$299.00
In stock
SKU
KE00989
| Product name | Human TIGAR ELISA Kit |
| Synonyms | C12orf5, EC:3.1.3.46, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TP53-induced glycolysis regulatory phosphatase |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 57103 |
| Sample Type | Average Range |
| Cell lysate | 96% 90%-105% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 5.14 0.18 3.56 |
| 2 | 16 1.36 0.03 2.17 |
| 3 | 16 0.73 0.03 3.44 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
TP53-induced glycolysis and apoptosis regulator (TIGAR) is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis. It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival. TIGAR can aggravate myocardial ischemia injury and heart failure in animal and cell models. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms C12orf5, EC:3.1.3.46, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TP53-induced glycolysis regulatory phosphatase FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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