Human/Mouse PER1 ELISA Kit KE00743 proteintech
$299.00
In stock
SKU
KE00743
| Product name | Human/Mouse PER1 ELISA Kit |
| Synonyms | hPER, hPER1, KIAA0482, PER, period homolog 1 (Drosophila) |
| Reactivity | Human/Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 15.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5187 |
| Sample Type | Average Range |
| Cell lysate | 102% 101%-105% |
| Tissue lysate | 89% 84%-95% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 2,550.3 114.8 4.5 |
| 2 | 16 593.7 39.6 6.7 |
| 3 | 16 295.9 15.0 5.1 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
PER1 (Period Circadian Clock 1) is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms hPER, hPER1, KIAA0482, PER, period homolog 1 (Drosophila) FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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