MagBind Cell Total RNA Extraction Kit (Prepackaged 48T) servicebio G3694-48T
$50.00
In stock
SKU
G3694-48T
Product Information
| Product Name | Cat.No. |
| Spec. | MagBind Cell Total RNA Extraction Kit (Prepackaged 48T) |
| G3694-48T | 48T |
Description/Introduction
This product is 48 prepackaged plates, which uses Servicebio Y-16, Y-48 automatic nucleic acids extractor and self-developed superparamagnetic magnetic beads to have strong reversible adsorption of nucleic acids under certain buffer conditions. It is designed for total RNA extraction of 48 samples from 1×105-1×107 fresh or cryopreserved cells, and the entire process can be completed in less than 1 hour, replacing the current time-consuming and cumbersome ethanol precipitation steps and a large number of rinsing steps. DNase contains visual dyes that have a sample tracing effect, preventing DNase from being missed and resulting in genomic DNA residue in the extracted RNA. The extracted RNA can be directly used in various molecular biology experiments such as RT-PCR, RT-qPCR, Northern hybridization, in vitro translation, RNase protection analysis and molecular cloning.This product is a pre-assembled panel of 48.，The Servicebio Y-16 and Y-48 fully automatic nucleic acid extractors, along with self-developed super paramagnetic beads, are designed to reversibly adsorb nucleic acids under certain buffer conditions. They are used for the simultaneous extraction of RNA from up to 48 samples of various fresh or frozen animal tissues.，The entire process is completed within 1 hour.Can replace the current time-consuming and cumbersome ethanol precipitation steps as well as numerous washing steps.。DNase contains a visualization dye with a loading tracking function, preventing the omission of DNase addition, which could lead to the presence of genome DNA residues in the extracted RNA.。The extracted RNA can be directly used for various molecular biology experiments such as RT-PCR, RT-qPCR, Northern hybridization, in vitro translation, RNase protection analysis, and molecular cloning.
Storage and Handling Conditions
DNase, Proteinase K, DTT Solution shipped with wet ice and stored at -20°C. Other reagents were shipped and stored at room temperature; valid for up to 12 months.
Product Contents
| Component Number | Component |
| G3694-48T | G3694-1 |
| Buffer RL | 30 mL |
| G3694-2 | DTT Solution |
| 1.2 mL | G3694-3 |
| Proteinase K | 500 µL |
| G3694-4 | DNase |
| 500 µL | G3694-5 |
| 96-well prepackaged plate | 3 |
| G3694-6 | 8-hole magnetic rod sleeve |
| 6 | Manual |
| 1 copy |
Before starting (please read carefully)
1. Check if there is any leakage of the prepackaged plates and discrepancy in the instruction manual. If such a phenomenon occurs, do not use it.
2. Before use, observe whether the magnetic beads or reagents are stuck to the sealing film or the hole wall of the reagent prepackaged plates. If this phenomenon occurs, you can mix them up and down and then centrifuge briefly or gently tap the bottom of the prepackaged plates on the table until the droplets fall off before use.
3. If a precipitate has formed in Buffer RL, warm it at 37°C until the precipitate has fully dissolved. Use it after cooling down to room temperature.
4. Before operation, add appropriate volume of DTT Solution into Buffer RL to a final concentration of 4%. Add 40 µL DTT Solution per 1 mL of Buffer RL. It should be prepared immediately before use. This mixture can be stored at 4℃ for one month.
5. Add 10 µL of DNase (blue) to the liquid (yellow) in the 4th and 10th columns of the prepackaged plates, and the liquid will be green after adding DNase.
Assay Protocol / Procedures
1. Collecting cell:
a. Collection of suspended cells: Take 105-107 cell suspension in a 1.5 mL centrifuge tube, centrifuge at 1,000 rpm for 5 min and gently discard supernatant using a pipette.
b. Collection of adherent cells: Discard the cell culture medium, add trypsin to treat the cells, take a 105-107 cell suspension, place it in a 1.5 mL centrifuge tube, centrifuge at 1,000 rpm for 5 min, and gently aspirate the supernatant using a pipette.
2. Add 500 µL Buffer RL to 1×105-1×107 fresh or frozen cells (please confirm that DTT Solution was added before use), blow with pipette repeatedly until no obvious precipitation is found, then add 10 µL Proteinase K, mix and incubate at 56℃ for 15 min;
3. Preparation of prepackaged plates: Take out the prepackaged plate, centrifuge it at 500 rpm for 1min, or gently tap the bottom of the prepackaged plate on the table until the liquid drops off, and then carefully tear off the aluminum foil sealing film;
4. Transfer the mixture in step 2 to the 1th and 7th columns of the prepackaged plates;
5. Place the prepackaged plates on the corresponding position of the nucleic acid extractor;
6. Parameter settings of Servicebio Y-16 and Y-48 automatic nucleic acid extractor:
| Step | First |
| Second | Third |
| Fourth | Fifth |
| Sixth | Seventh |
| Eighth | Ninth |
| Station | 3 |
| 1 | 2 |
| 3 | 4 |
| 3 | 5 |
| 6 | 5 |
| Wait Time | 00:00:00 |
| 00:00:00 | 00:00:00 |
| 00:00:00 | 00:03:00 |
| 00:00:00 | 00:00:00 |
| 00:05:00 | 00:00:00 |
| Mix Modea | 2 |
| 2 | 2 |
| 2 | 2 |
| 2 | 2 |
| 2 | 2 |
| Mix Time | 00:00:10 |
| 00:05:00 | 00:01:00 |
| 00:01:00 | 00:10:00 |
| 00:01:00 | 00:01:00 |
| 00:05:00 | 00:00:10 |
| Pause | Ⓧ |
| Ⓧ | Ⓧ |
| Ⓧ | Ⓧ |
| Ⓧ | Ⓧ |
| Ⓧ | Ⓧ |
| Mag Timea | 00:00:30 |
| 00:00:30 | 00:00:30 |
| 00:00:30 | 00:00:30 |
| 00:00:30 | 00:00:30 |
| 00:01:00 | 00:00:00 |
| Volume(µL) | 600 |
| 800 | 500 |
| 600 | 100 |
| 600 | 600 |
| 80 | 600 |
| Temperature(℃) | --- |
| 25.0℃ | --- |
| --- | --- |
| --- | --- |
| --- | --- |
a: If you do not use the Servicebio Y-16 and Y-48 automatic nucleic acid extractor, you can adjust the magnetic attracting time and mixing speed parameters according to other instrument instructions suitable for this product.
7. Install the magnetic sleeve, run the program.;
8. After the automated extraction steps are completed,, transfer the nucleic acid solution from the 6th and 12th columns to a new Nuclease-free centrifuge tube carefully and store at -80℃ for long-term storage. carefully transfer the nucleic acid solution from the wells in column 6 and column 12 to new Nuclease-free centrifuge tubes and store them at -80°C for long-term preservation.。
Note
1. Please read this product manual carefully before operating.。
2. Please do not invert the prepackaged plates.
3. Do not install the board upside down.。
4. Do not dry the magnetic beads for a long time to avoid affecting the efficiency of RNA elution.
5. The samples frozen at low temperature should avoid repeated freezing and thawing, so as not to affect the integrity of RNA.
6. For your safety and health, please wear lab clothes, disposable gloves, a mask, avoid speaking, and use a Nuclease free tips and centrifuge tubes to avoid cross-contamination.
For Research Use Only!
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