MagBind Blood Total RNA Extraction Kit (Prepackaged 48T) servicebio G3692-48T
$50.00
In stock
SKU
G3692-48T
Product Information
| Product Name | Cat.No. |
| Spec. | MagBind Blood Total RNA Extraction Kit (Prepackaged 48T) |
| G3692-48T | 48T |
Description/Introduction
This product is 48 prepackaged plates, which uses Servicebio Y-16, Y-48 automatic nucleic acids extractor and self-developed superparamagnetic magnetic beads to have strong reversible adsorption of nucleic acids under certain buffer conditions. It is designed for RNA extraction from 48 samples of fresh blood preserved with various anticoagulants in volumes less than 1.5 mL. This product can completely remove contaminants and enzyme inhibitors from the blood, making total RNA isolation fast, convenient and reliable, the whole process is completed within 1 hour, replacing the current time-consuming and cumbersome ethanol precipitation steps and extensive rinsing steps. DNase contains visual dye, which has the function of sample addition and tracer to prevent DNase from missing addition, resulting in genomic DNA residue in the extracted RNA. The extracted RNA can be directly used for various molecular biology experiments, including RT-PCR, RT-qPCR, Northern hybridization, in vitro translation, RNase protection analysis, and molecular cloning.
Storage and Handling Conditions
DNase, DTT Solution shipped with wet ice and stored at -20°C. Other reagents were shipped and stored at room temperature; valid for up to 12 months.
Product Contents
| Component Number | Component |
| G3692-48T | G3692-1 |
| 10×Red Cell Lysis Buffer | 60 mL |
| G3692-2 | Buffer BRL |
| 30 mL | G3692-3 |
| DTT Solution | 1.2 mL |
| G3692-4 | DNase |
| 500 µL | G3692-5 |
| 96-well prepackaged plate | 3 |
| G3692-6 | 8-hole magnetic rod sleeve |
| 6 | Manual |
| 1 copy |
Before starting (please read carefully)
1. Check if there is any leakage of the prepackaged plates and discrepancy in the instruction manual. If such a phenomenon occurs, do not use it.
2. Before use, observe whether the magnetic beads or reagents are stuck to the sealing film or the hole wall of the reagent prepackaged plates. If this phenomenon occurs, you can mix them up and down and then centrifuge briefly or gently tap the bottom of the prepackaged plates on the table until the droplets fall off before use.
3. If a precipitate has formed in Buffer BRL, warm it at 37°C until the precipitate has fully dissolved. Use it after cooling down to room temperature.
4. Before operation, add appropriate volume of DTT Solution into Buffer BRL to a final concentration of 4%. Add 40 µL DTT Solution per 1 mL of Buffer BRL. It should be prepared immediately before use. This mixture can be stored at 4℃ for one month.
5. Add 10 µL of DNase (blue) to the liquid (yellow) in the 4th and 10th columns of the prepackaged plates, and the liquid will be green after adding DNase.
6. Please calculate the required amount in advance and dilute 10×Red Cell Lysis Buffer to 1×Red Cell Lysis Buffer before use.
Assay Protocol / Procedures
1. Sample lysis:
a. Take 50 µL-1.5 mL anucleated red cell whole blood (containing anticoagulant) in a centrifuge tube. Add 5 volume of 1×Red Cell Lysis Buffer. Mix it upside down and leave it at room temperature for 5 min, during which time mix it upside down for 2-3 times, centrifuge it at 3,000 rpm for 10 min at 4℃, gently absorb and discard the supernatant with a pipette, do not absorb precipitation (If the blood sample is a frozen sample, repeat the procedure using 1×Red Cell Lysis Buffer until the precipitate turns white. If some red blood cells or cell fragments were still visible along with the leucocyte granules, add 2 volume of 1×Red Cell Lysis Buffer suspend leucocyte, and incubate them at room temperature for 5 min, centrifuge at 3,000 rpm for 10 min at 4℃, and remove the supernatant completely).
b. Take 5-20 μL nucleated red cell whole blood (including anticoagulant) and place it in 1.5 mL centrifuge tube for the next step.
2. Add 500 µL Buffer BRL (please confirm that DTT Solution has been added before use), vortex or blow evenly with a pipette until there is no obvious leukocyte precipitation;
3. Preparation of prepackaged plates: Take out the prepackaged plate, centrifuge it at 500 rpm for 1min, or gently tap the bottom of the prepackaged plate on the table until the liquid drops off, and then carefully tear off the aluminum foil sealing film;
4. Transfer all the reagents from step 2 to the 1th and 7th columns of the prepackaged plates;
5. Place the prepackaged plates on the corresponding position of the nucleic acid extractor;
6. Parameter settings of Servicebio Y-16 and Y-48 automatic nucleic acid extractor:
| Step | First |
| Second | Third |
| Fourth | Fifth |
| Sixth | Seventh |
| Eighth | Ninth |
| Station | 3 |
| 1 | 2 |
| 3 | 4 |
| 3 | 5 |
| 6 | 5 |
| Wait Time | 00:00:00 |
| 00:00:00 | 00:00:00 |
| 00:00:00 | 00:03:00 |
| 00:00:00 | 00:00:00 |
| 00:05:00 | 00:00:00 |
| Mix Modea | 2 |
| 2 | 2 |
| 2 | 2 |
| 2 | 2 |
| 2 | 2 |
| Mix Time | 00:00:10 |
| 00:05:00 | 00:01:00 |
| 00:01:00 | 00:10:00 |
| 00:01:00 | 00:01:00 |
| 00:05:00 | 00:00:10 |
| Pause | Ⓧ |
| Ⓧ | Ⓧ |
| Ⓧ | Ⓧ |
| Ⓧ | Ⓧ |
| Ⓧ | Ⓧ |
| Mag Timea | 00:00:30 |
| 00:00:30 | 00:00:30 |
| 00:00:30 | 00:00:30 |
| 00:00:30 | 00:00:30 |
| 00:01:00 | 00:00:00 |
| Volume(µL) | 600 |
| 800 | 500 |
| 600 | 100 |
| 600 | 600 |
| 50 | 600 |
| Temperature(℃) | --- |
| 25.0℃ | --- |
| --- | --- |
| --- | --- |
| --- | --- |
a: If you do not use the Servicebio Y-16 and Y-48 automatic nucleic acid extractor, you can adjust the magnetic attracting time and mixing speed parameters according to other instrument instructions suitable for this product.
7. Install the magnetic sleeve, run the program.;
8. After the automated extraction steps are completed,, transfer the nucleic acid solution from the 6th and 12th columns to a new Nuclease-free centrifuge tube carefully and store at -80℃ for long-term storage. carefully transfer the nucleic acid solution from the wells in column 6 and column 12 to new Nuclease-free centrifuge tubes and store them at -80°C for long-term preservation.。
Note
1. Please read this product manual carefully before operating.。
2. Please do not invert the prepackaged plates.
3. Do not install the board upside down.。
4. Do not dry the magnetic beads for a long time to avoid affecting the efficiency of RNA elution.
5. For your safety and health, please wear lab clothes, disposable gloves, a mask, avoid speaking, and use a Nuclease free tips and centrifuge tubes to avoid cross-contamination.
For Research Use Only!
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