Recombinant Human PDL1(CD274) Protein (His-tag) servicebio GB300602-H-10μg
$50.00
In stock
SKU
GB300602-H-10娓璯
DESCRIPTION
SPECIFICATIONS
STORAGE
BACKGROUND
PD-L1, which we first identified as a homologue of B7 family molecules, is expressed on immune cells, i.e., dendritic cells (DCs) and B and T cells, and controls T-cell immune responses such as peripheral tolerance, termination of immune responses and immune exhaustion after prolonged exposure to an antigen stimulus. PD-L1 plays a crucial role in the disease progression of multiple myeloma. PD-L1 has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1 can bind to PD-1 expressed on cytotoxic T lymphocytes (CTLs), resulting in the escape from immune surveillance by the induction of CTL apoptosis. PD-L1 is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
Data
| Gene Name Synonym | PDL1, CD274, B7H1, PDCD1L1, PDCD1LG1 |
| Species | Human |
| Accession | Q9NZQ7 |
| Source | HEK293 cells |
| Amino acid sequence | >sp|Q9NZQ7|19-238 is expressed with a 10×His tag at the N terminus |
| Molecular Weight | 25.2 kDa |
| Purity | >95.0% as determined by SDS-PAGE |
| Endotoxin | <5 EU mg -1 |
| Formulation | Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. |
| Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. |
| Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
| SDS-PAGE | Bioactivity |
| Active Recombinant Human PDL1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-40 kDa. | Measured by its binding ability in a functional ELISA. Immobilized human PDL1 at 3 μg/ml (100 μl /well) can bind biotinylated human PD1 with a linear range of 0.039-0.652 μg/mL. |
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