Recombinant Human IL-5 Protein (His-tag) servicebio GB300006-H-500μg
$50.00
In stock
SKU
GB300006-H-500娓璯
DESCRIPTION
SPECIFICATIONS
STORAGE
BACKGROUND
IL-5 critically regulates expression of genes involved in cell survival, IgH switch recombination, maturation in B cells and genes required for growth, survival, and effector function of eosinophils. IL-5 was recognized as the major maturation and differentiation factor for eosinophils in mice and humans. IL-5 was originally defined as a T-cell-derived cytokine that triggers activated B cells for terminal differentiation into antibody-secreting plasma cells. IL-5 critically regulates expression of genes involved in proliferation, cell survival and maturation and effector functions of B cells and eosinophils. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. Interleukin 5 has long been associated with several allergic diseases, including allergic rhinitis and asthma.
Data
| Gene Name Synonym | IL-5, IL5, TRF, IL5, Interleukin-5 |
| Species | Human |
| A ccession | P05113 |
| Source | HEK293 cells |
| Amino acid sequence | Asn49-Ser162 is expressed with a 10×His tag at the C terminus |
| Molecular Weight | 14.4 kDa |
| Purity | >95.0% as determined by SDS-PAGE |
| Endotoxin | <5 EU mg -1 |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. |
| Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. |
| Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
| SDS-PAGE | Bioactivity |
| Active Recombinant Human IL-5 Protein was determined by SDS-PAGE with Coomassie Blue. | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-1 ng/mL. |
For Research Use Only!
Ver. No.: V1.0-202102
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