Recombinant Rat IL-10 Protein (His-tag) servicebio GB300011-R-500μg
$50.00
In stock
SKU
GB300011-R-500娓璯
DESCRIPTION
SPECIFICATIONS
STORAGE
BACKGROUND
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that belongs to the IL-10 family.The Interleukin (IL)-10 cytokine family includes IL-10, IL-19, IL-20, IL-22, IL-24, and IL-26, which are considered as Class 2α-helical cytokines. IL-10, a cytokine with anti-inflammatory properties, has a central role in infection by limiting the immune response to pathogens and thereby preventing damage to the host. IL10 is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes.
Data
| Gene Name Synonym | IL-10, Il10, Il-10 |
| Species | Rat |
| Accession | P29456 |
| Source | HEK293 cells |
| Amino acid sequence | Met19-Asn178 is expressed with a 6×His tag at the C terminus |
| Molecular Weight | 21.6 kDa |
| Purity | >95.0% as determined by SDS-PAGE |
| Endotoxin | <5 EU mg -1 |
| Formulation | Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. |
| Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. |
| Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
| SDS-PAGE | Bioactivity |
| Active Recombinant Rat IL-10 Protein was determined by SDS-PAGE with Coomassie Blue. | Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is 3-8 ng/mL. |
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