S-Vision Poly-HRP Conjugated Goat Anti-Rabbit IgG (H+L), Ready to Use servicebio G1302-10ML
$50.00
In stock
SKU
G1302-10ML
Product Information
| Product Name: S-Vision Poly-HRP Conjugated Goat Anti- Rabbit IgG (H+L) , Ready to Use | Cat. No .: G1302 - 10 ML |
| Spec.: 10 mL | G1302 - 100 ML |
| 100 mL |
Product Description
This product utilize a dextran backbone to which multiple HRP enzyme and antibodies molecules are conjugated. This polymer creates a highly sensitive, readily available and one-step non-biotin detection system for immunohistochemical and immunocytochemical staining.This system avoids the use of streptavidin and biotin, and therefore eliminates non-specific staining as a result of endogenous biotin..It has a wide adaptability to sample processing, reducing the variability caused by different tissue processing methods.The reagent is ready-to-use, which does not need to be diluted to obtain the best dyeing ratio, reducing experimental operation errors.
Storage and Shipping Conditions
Wet ice packs for transportation, store at 2-8℃ for 12 months.
Product Content
| Component Number: G1302 | Component: S-Vision Poly-HRP Conjugated Goat Anti- Rabbit IgG (H+L) , Ready to Use |
| G1302 - 10 ML: 10 mL | G1302 - 100 ML: 100 mL |
| Product Manual | 1 pc |
The Staining Process
1. Manually IHC Staining
| Procedures: D ewax | R eagent: BioDewax and Clear Solution ( Recommendation G1128 ) |
| Protocol: The sections were placed in BioDewax and Clear SolutionⅠ10 min -BioDewax and Clear Solution Ⅱ10 m in -BioDewax and Clear Solution Ⅲ10 m in - absolute ethanol Ⅰ5 m in - absolute ethanol Ⅱ5 m in - absolute ethanol Ⅲ 5 m in - wash in pure water . | Procedures: Antigen Retrieval |
| R eagent: Citrate Antigen Retrieval Solution pH6.0 ( Recommendation G1202 )o r EDTA Antigen Retrieval Solution pH 9.0 ( Recommendation G1203 ) | Protocol: Microwave Heating : H eating to boiling, maintain a temperature above 98℃ for 15-20 min , and cool ing to room temperature ; P ressure cooker heating : Heat in the pressure cooker to air injection for 2 min ; N ote : The repair time is also related to the fixed time and the antigen epitope, so the conditions can be explored . |
| Procedures: Blocking of the endogenous peroxidases | R eagent: 3% Hydrogen peroxide |
| Protocol: Dipping or incubate at room temperature for 10 min . Wash the slide in PBS (pH 7.4) on a shaker 3 times for 5 min each time . | Procedures: B locking |
| R eagent: BSA ( Recommendation GC305010 ) | Protocol: The 3% BSA working solution was prepared, and the tissue was completely covered by drops, and incubated at room temperature for 30 min . |
| Procedures: P rimar y antibody | R eagent: commercialization P rimar y antibody |
| Protocol: Add a working solution to completely cover the tissue, usually 100 μL, and incubate at 4℃ overnight or 37℃ for 2 hours . | Procedures: S econdary antibody |
| R eagent: S-vision poly-HRP conjugated antibody | Protocol: Drop this product until tissue is completely covered, usually 100 μL, incubated at room temperature for 20 min . |
| Procedures: DAB staining | R eagent: DAB Chromogenic Kit ( Recommendation G1212 ) |
| Protocol: Add the prepared DAB working solution to completely cover the tissue and develop color for 5-10 min . | Procedures: H ematoxylin staining |
| R eagent: Hematoxylin Solution ( Recommendation G1004 ) hematoxylin differentiation solution ( Recommendation G1039 ) hematoxylin bluing solution ( Recommendation G1040) | Protocol: The sections were directly stained into hematoxylin staining solution for 3-5 min and washed with water; then stained by hematoxylin differentiation solution for 2-5 s and washed with water; hematoxylin bluing solution for 1min and washed with water . |
| Procedures: Dehydration and Mounti ng | R eagent: Mounting Medium |
| Protocol: 75% alcohol 5 m in -85% alcohol 5 m in - absolute ethanol Ⅰ5 m in - absolute ethanol Ⅱ5 m in - n-butyl alcohol 5 m in -xylene Ⅰ5 min , The section were removed from xylene and mounting . |
2. Automation in IHC
This protocol uses the Servicebio IHC-48 histochemical instrument for reference only. For other instruments, refer to the instructions for each instrument.
| Procedures: D ewax | R eagent: BioDewax and Clear Solution ( Recommendation G1128 ) |
| Protocol: Dewax at 62℃ for 3 min , repeated three times , Wash with absolute ethanol for 3 times and wash twice with pure water . | Procedures: Antigen Retrieval |
| R eagent: Antigen Retrieval Solution (For histochemical instrument) | Protocol: Antigen Retrieval Solution (For histochemical instrument) , Heat at 100℃ for 25 min and cool for 20 min . |
| Procedures: Blocking of the endogenous peroxidases | R eagent: 3% Hydrogen peroxide |
| Protocol: I ncubate at room temperature for 10 min . | Procedures: B locking |
| R eagent: BSA ( Recommendation GC305010 ) | Protocol: The 3% BSA working solution was prepared, and incubated at room temperature for 1 0 min . |
| Procedures: P rimar y antibody | R eagent: commercialization P rimar y antibody |
| Protocol: W orking solution was incubated at 37℃ for 15 – 30 min and washed three times with PBS . | Procedures: S econdary antibody |
| R eagent: S-vision poly-HRP conjugated antibody | Protocol: incubated at RT for 20 min and washed three times with PBS . |
| Procedures: DAB staining | R eagent: DAB Substrate Kit ( Recommendation G1212 ) |
| Protocol: Add the prepared DAB working solution and develop color for 5-10 min, washed three times with water. | Procedures: H ematoxylin staining |
| R eagent: Mayer Hematoxylin Solution | Protocol: I ncubate at room temperature for 10 min . , washed three times with water. |
| Procedures: Dehydration and Mounti ng | R eagent: Mounting Medium |
| Protocol: 75% alcohol 5 m in -85% alcohol 5 m in - absolute ethanol Ⅰ5 m in - absolute ethanol Ⅱ5 m in - n-butyl alcohol 5 m in -xylene Ⅰ5 min , The section were removed from xylene and mounting . |
Note
1. This product is a ready-to-use reagent without dilution.
2. If the background is deep, the primary antibody can be diluted by gradient to obtain better results.,
3. Wear protective equipment. for your safety
For Research Use Only!
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