S-Vision Poly-HRP Conjugated Goat Anti-Mouse IgG (H+L), Ready to Use servicebio G1301-100ML
$50.00
In stock
SKU
G1301-100ML
ProductInformation
| Product Name: S-Vision Poly-HRP Conjugated Goat Anti-Mouse IgG (H+L) , Ready to Use | Cat. No: G1301- 10 ML |
| Spec.: 10 mL | G1301- 100 ML |
| 100 mL |
ProductDescription
This product utilizes a dextran backbone to which multiple HRP enzyme and antibodies molecules are conjugated. This polymer creates a highly sensitive, readily available and one-step non-biotin detection system for immunohistochemical and immunocytochemical staining. This system avoids the use of streptavidin and biotin and therefore eliminates non-specific staining as a result of endogenous biotin. It has a wide adaptability to sample processing, reducing the variability caused by different tissue processing methods. The reagent is ready-to-use, which does not need to be diluted to obtain the best dyeing ratio, reducing experimental operation errors.
StorageandShippingConditions
Wet ice packs for transportation, store at 2-8℃ for 12 months.
ProductContent
| Component Number: G1301 | Component: S-Vision Poly-HRP Conjugated Goat Anti-Mouse IgG (H+L) , Ready to Use |
| G1301- 10 ML: 10 mL | G1301- 100 ML: 100 mL |
| Product Manual | One copy |
TheStainingProcess
1. ManuallyIHCStaining
| Procedures: D ewax | R eagent: BioDewax and Clear Solution ( Recommendation G1128 ) |
| Protocol: The sections were placed in BioDewax and Clear SolutionⅠ10 min -BioDewax and Clear Solution Ⅱ10 m in -BioDewax and Clear Solution Ⅲ10 m in - absolute ethanol Ⅰ5 m in - absolute ethanol Ⅱ5 m in - absolute ethanol Ⅲ 5 m in - wash in pure water . | Procedures: Antigen Retrieval |
| R eagent: Citrate Antigen Retrieval Solution pH6.0 ( Recommendation G1202 ) o r EDTA Antigen Retrieval Solution pH 9.0 ( Recommendation G1203 ) | Protocol: Microwave Heating : H eating to boiling, maintain a temperature above 98℃ for 15-20 min , and cool ing to room temperature ; P ressure cooker heating : Heat in the pressure cooker to air injection for 2 min ; N ote : The repair time is also related to the fixed time and the antigen epitope, so the conditions can be explored . |
| Procedures: Blocking of the endogenous peroxidases | R eagent: 3% Hydrogen peroxide |
| Protocol: Dipping or incubate at room temperature for 10 min . Wash the slide in PBS (pH 7.4) on a shaker 3 times for 5 min each time . | Procedures: B locking |
| R eagent: BSA ( Recommendation GC305010 ) | Protocol: The 3% BSA working solution was prepared, and the tissue was completely covered by drops, and incubated at room temperature for 30 min . |
| Procedures: P rimar y antibody | R eagent: commercialization P rimar y antibody |
| Protocol: Add a working solution to completely cover the tissue, usually 100 μL, and incubate at 4℃ overnight or 37℃ for 2 hours . | Procedures: S econdary antibody |
| R eagent: S-vision poly-HRP conjugated antibody | Protocol: Drop this product until tissue is completely covered, usually 100 μL, incubated at room temperature for 20 min . |
| Procedures: DAB staining | R eagent: DAB Chromogenic Kit ( Recommendation G1212 ) |
| Protocol: Add the prepared DAB working solution to completely cover the tissue and develop color for 5-10 min . | Procedures: H ematoxylin staining |
| R eagent: Hematoxylin Solution ( Recommendation G1004 ) hematoxylin differentiation solution ( Recommendation G1039 ) hematoxylin bluing solution ( Recommendation G1040) | Protocol: The sections were directly stained into hematoxylin staining solution for 3-5 min and washed with water; then stained by hematoxylin differentiation solution for 2-5 s and washed with water; hematoxylin bluing solution for 1min and washed with water . |
| Procedures: Dehydration and Mounti ng | R eagent: Mounting Medium |
| Protocol: 75% alcohol 5 m in -85% alcohol 5 m in - absolute ethanol Ⅰ5 m in - absolute ethanol Ⅱ5 m in - n-butyl alcohol 5 m in -xylene Ⅰ5 min , The section were removed from xylene and mounting . |
2. AutomationinIHC
This protocol uses the Servicebio IHC-48 histochemical instrument for reference only. For other instruments, refer to the instructions for each instrument.
| Procedures: D ewax | R eagent: BioDewax and Clear Solution ( Recommendation G1128 ) |
| Protocol: Dewax at 62℃ for 3 min , repeated three times , Wash with absolute ethanol for 3 times and wash twice with pure water . | Procedures: Antigen Retrieval |
| R eagent: Antigen Retrieval Solution (For histochemical instrument) | Protocol: Antigen Retrieval Solution (For histochemical instrument) , Heat at 100℃ for 25 min and cool for 20 min . |
| Procedures: Blocking of the endogenous peroxidases | R eagent: 3% Hydrogen peroxide |
| Protocol: I ncubate at room temperature for 10 min . | Procedures: B locking |
| R eagent: BSA ( Recommendation GC305010 ) | Protocol: The 3% BSA working solution was prepared, and incubated at room temperature for 1 0 min . |
| Procedures: P rimar y antibody | R eagent: commercialization P rimar y antibody |
| Protocol: W orking solution was incubated at 37℃ for 15 – 30 min and washed three times with PBS . | Procedures: S econdary antibody |
| R eagent: S-vision poly-HRP conjugated antibody | Protocol: incubated at RT for 20 min and washed three times with PBS . |
| Procedures: DAB staining | R eagent: DAB Substrate Kit ( Recommendation G1212 ) |
| Protocol: Add the prepared DAB working solution and develop color for 5-10 min , washed three times with water. | Procedures: H ematoxylin staining |
| R eagent: Mayer Hematoxylin Solution | Protocol: I ncubate at room tempe rature for 10 min . , washed three times with water. |
| Procedures: Dehydration and Mounti ng | R eagent: Mounting Medium |
| Protocol: 75% alcohol 5 m in -85% alcohol 5 m in - absolute ethanol Ⅰ5 m in - absolute ethanol Ⅱ5 m in - n-butyl alcohol 5 m in -xylene Ⅰ5 min , The section were removed from xylene and mounting . |
Note
1. This product is a ready-to-use reagent without dilution.
2. If the background is deep, the primary antibody can be diluted by gradient to obtain better results.,
3. Wear protective equipment. for your safety
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