Product Information
| Protein full name | Cyclic AMP-dependent transcription factor ATF-4 |
| Synonyms | cAMP-dependent transcription factor ATF-4, Activating transcription factor 4, C/EBP-related ATF, C/ATF, TaxREB67 homolog, Atf4 |
| Uniprot ID | Q06507 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse ATF-4 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 38 kDa / 45 kDa |
| Purity | Affinity purification |
Applications
| Applications: WB | Species: Mouse, Rat |
| Dilution: 1: 500-1: 1000 | Positive Tissue: brain, retina |
Background
Activating transcription factor 4 (tax-responsive enhancer element B67), also known as ATF4, is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The protein is characterized as the cAMP-response element binding protein 2 (CREB-2) and belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. ATF4 transcription factor is also known to play role in osteoblast differentiation along with RUNX2 and osterix.
Images
WB analysis of ATF-4(GB11813). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).