Anti-c-Fos Mouse mAb servicebio GB12069-100
$50.00
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SKU
GB12069-100
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The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
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| Protein full name | Protein c-Fos |
| Synonyms | FOS, AP-1, C-p55, Fos proto-oncogene, AP-1 transcription factor subunit |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Human c-Fos |
| Uniprot ID | P01100 |
| Isotype | IgG1, κ |
| Clone Number | 4B4F11 |
| Purity | Affinity purification |
| Subcellular location | Nucleus, Endoplasmic reticulum, Cytoplasm |
| Predicted MW./Observed MW. | 41 kDa / 50-65 kDa |
| Applications | Species |
| Dilution | Positive Tissue |
| WB | Human, Mouse, Rat |
| 1: 500-1: 2000 | kidney, brain, lung |
| IHC/IF | Human, Mouse, Rat |
| 1: 200-1: 2000 | brain |
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
| WB analysis of c-Fos(GB12069). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h. | IHC analysis of c-Fos(GB12069). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IF analysis of c-Fos(GB12069)red. Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
For Research Use Only! Ver. No.: V2.1-20260206
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