MEM, no L-Glutamine, no HEPES servicebio G4553-500ML
$50.00
In stock
SKU
G4553-500ML
Product Information
| Product Name: MEM, no L-Glutamine, no HEPES | Cat. No.: G 4553-500ML |
| Spec.: 500 mL |
Product Description/Introduction
MEM (Minimum Essential Medium) is one of the most commonly used basal medium for supporting the growth of many suspension and adherent mammalian cells. MEM medium is a more nutrient rich version of basal medium eagle (BME) media. There are some other modifications of MEM followed, including with or without non-essential amino acids (including L-Alanine, L-Glutamic acid, L-Asparagine, L-Aspartic acid, L-Proline, L-Serine, and Glycine), addition of ribonucleosides and deoxyribonucleosides. MEM is available with Earle’s salts for use in a CO2 incubator or with Hank’s salts for use without CO2. This product is made with Earle’s salts, no addition of non-essential amino acids and ribonucleosides and deoxyribonucleosides.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM with Earle’s salts uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. MEM with Hanks’ salts does not require a CO2 environment to maintain physiological pH.
All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.
The major character of this MEM is as follows:
l With 5.5 mM Glucose, phenol red
l Without non-essential amino acid (NEAA), L-glutamine, sodium pyruvate, HEPES.
Storage and Shipping Conditions
Ship at room temperature; Store at 2℃ to 8℃, protect from light; Valid for 12 months.
Note
1. This product is filtered by 0.1μm to remove bacteria. Please pay attention to aseptic operation to avoid contamination.
2. For best results, do not repeat freeze-thaw cycles.
3. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
G4553 MEM medium
Vitamins
| Components | Concentration |
| Components | Concentration |
| mg/L | mM |
| mg/L | mM |
| Amino acids | Inorganic salts |
| L-A rginine hydrochloride | 126.0 |
| 0.5972 | C alcium Chloride (CaCl 2 ) (anhyd.) |
| 200 | 1.802 |
| L-Cystine 2HCl | 31.0 |
| 0.09904 | Magnesium Sulfate (MgSO 4 ) (anhyd.) |
| 200 | 0.81 3 |
| L-Glutamine | 292.0 |
| 2.0 | Potassium Chloride (KCl) |
| 400 | 5.333 |
| L-Histidine hydrochloride-H2O | 42.0 |
| 0.20 | Sodium Bicarbonate (NaHCO 3 ) |
| 2200 | 26.19 |
| L-Isoleucine | 52.0 |
| 0.3969 | Sodium Chloride (NaCl) |
| 6800 | 117.2 |
| L-Leucine | 52.0 |
| 0.3969 | Sodium Phosphate monobasic ( NaH 2 PO 4 ) (anhyd.) |
| 122 | 1.014 |
| L-Lysine hydrochloride | 73.0 |
| 0.3989 | Other components |
| L-Methionine | 15.0 |
| 0.1007 | D-Glucose (Dextrose) |
| 1000 | 5.556 |
| L-Phenylalanine | 32.0 |
| 0.1939 | Phenol Red |
| 9 .4 | 0.02657 |
| L- T hreonine | 48.0 |
| 0.4034 | H EPES |
| – | – |
| L- T ryptophan | 10.0 |
| 0.04902 | L- T yrosine disodium salt dihydrate |
| 52.0 | 0.1992 |
| L-Valine | 46.0 |
| 0.3932 | Choline chloride |
| 1.0 | 0.007143 |
| D-Calcium pantothenate | 1.0 |
| 0.002096 | Folic Acid |
| 1.0 | 0.002268 |
| Niacinamide | 1.0 |
| 0.008197 | Pyridoxine hydrochloride |
| 1.0 | 0.004902 |
| Riboflavin | 0.1 |
| 0.0002660 | Thiamine hydrochloride |
| 1.0 | 0.002967 |
| i-Inositol | 2.0 |
| 0.01111 |
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