Ham's F-12, no L-Glutamine servicebio G4563-500ML
$50.00
In stock
SKU
G4563-500ML
Product Information
| Product Name: Ham's F-12, no L-Glutamine | Cat. No.: G4563-500ML |
| Spec.: 500 mL |
Product Description/Introduction
Ham's F-12 nutriment Mixture is based on Ham's F-10 nutriment mixture, and it is originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic medium for serum-free culture, especially suitable for single cell culture and clonal culture at low serum levels. It is also widely used in the culture of tumor cells and primary cells after adding serum, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts and chicken embryonic cells. In addition, Ham's F-12 can be mixed with DMEM in equal volumes to give a more nutritious DMEM/F-12 medium.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.
This product is sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 10 mM D-glucose, 1.0 mM sodium pyruvate, phenol red indicator, no HEPES buffer system and L-glutamine.
Storage and Shipping Conditions
Ship at room temperature; Store at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
Ham’s F-12 medium, no L-Glutamine Formula
Vitamins
| Components | Concentration |
| Components | Concentration |
| mg/L | mM |
| mg/L | mM |
| Amino acids | Inorganic salts |
| Glycine | 7.507 |
| 0.1 | Potassium Chloride (KCl) |
| 223.6 | 2.98 |
| L-Alanine | 8.90 |
| 0.10 | Sodium bicarbonate(NaHCO 3 ) |
| 1176 | 14 |
| L-Arginine monohydrochloride | 210.66 |
| 1.0 | Sodium chloride (NaCl) |
| 7599 | 131.02 |
| L-Asparagine monohydrate | 15.02 |
| 0.10 | Sodium Phosphate dibasic (Na 2 HPO 4 ) (anhyd.) |
| 141.96 | 1.0 |
| L-Asparagine | 13.31 |
| 0.1 | Calcium Chloride (CaCl 2 )(anhyd.) |
| 33.22 | 0.30 |
| L-Cysteine hydrochloride hydrate | 35.05 |
| 0.20 | Cupric Sulfate (CuSO 4 ·5H 2 O) |
| 0.0025 | 0.00001 |
| L-Glutamic acid | 14.71 |
| 0.1 | Ferrous Sulfate (FeSO 4 ·7H 2 O) |
| 0.834 | 0.003 |
| L-Glutamine | - |
| - | Magnesium chloride (MgCl 2 )(anhyd.) |
| 57.35 | 0.60 |
| L-Histidine hydrochloride monohydrate | 20.96 |
| 0.1 | Zinc Sulfate(ZnSO 4 ·7H 2 O) |
| 0.862 | 0.003 |
| L-Isoleucine | 4.005 |
| 0.031 | Other components |
| L-Leucine | 13.12 |
| 0.1 | D-Glucose (Dextrose) |
| 1802.0 | 10.01 |
| L-Lysinehydrochloride | 36.50 |
| 0.199 | Hypoxanthine |
| 1.13 | 0.00319 |
| L-Methionine | 4.51 |
| 0.03 | Lipoic acid |
| 110 | 1.0 |
| L-Phenylalanine | 5.01 |
| 0.03 | Phenol Red |
| 4.083 | 0.03 |
| L-Proline | 34.5 |
| 0.3 | Putrescine 2HCl |
| 0.084 | 0.0003 |
| L-Serine | 10.5 |
| 0.1 | Sodium pyruvate |
| 0.210 | 0.00102 |
| L-Threonine | 11.9 |
| 0.10 | Thymidine |
| 0.16107 | 0.001 |
| L-Tryptophan | 2.0 |
| 0.01 | D-Glucose (Dextrose) |
| 0.701 | 0.00289 |
| L-Tyrosine disodium salt | 7.8 |
| 0.0298 | L-Valine |
| 11.7 | 0.1 |
| Biotin | 0.0073 |
| 0.0000299 | Choline chloride |
| 14 | 0.1 |
| D-Calcium pantothenate | 0.50 |
| 0.001048 | Folic Acid |
| 1.30 | 0.002948 |
| Niacinamide | 0.04 |
| 0.000295 | Pyridoxine hydrochloride |
| 0.06 | 0.000291 |
| Riboflavin | 0.04 |
| 0.000098 | Thiamine hydrochloride |
| 0.30 | 0.000890 |
| Vitamin B12 | 1.40 |
| 0.001033 | i-Inositol |
| 18 | 0.1 |
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