Ham's F-12K, no L-Glutamine servicebio G4561-500ML
$50.00
In stock
SKU
G4561-500ML
Product Information
| Product Name: Ham's F-12K, no L-Glutamine | Cat. No.: G4561-500ML |
| Spec.: 500 mL |
Product Description/Introduction
Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment. Ham's F-12K improves the concentration of amino acid and pyruvate, reduces the content of glucose, and modifies the composition and content of inorganic salts on the basis of the original Ham's F-12. Compared with other media, Ham's F-12K contains many unique components, such as putrescine, thymidine, hypoxanthine, zinc, in addition to a higher content of sodium pyruvate.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, no protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.
This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 7.0 mM D-glucose, 2.0 mM sodium pyruvate, phenol red indicator, no HEPES buffer system and L-glutamine.
Storage and Shipping Conditions
Ship at room temperature; Store at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
Ham’s F-12K medium, no L-Glutamine Formula
Vitamins
| Components | Concentration |
| Components | Concentration |
| mg/L | mM |
| mg/L | mM |
| Amino acids | Inorganic salts |
| Glycine | 15 |
| 0.2 | Calcium Chloride (CaCl 2 )(anhyd.) |
| 102 | 0.9189 |
| L-Alanine | 18 |
| 0.2022 | Cupric Sulfate (CuSO 4 ·5H 2 O) |
| 0.002 | 0.0000080 |
| L-Arginine monohydrochloride | 422 |
| 2.0 | Ferrous Sulfate (FeSO 4 ·7H 2 O) |
| 0.8 | 0.002878 |
| L-Asparagine monohydrate | 30 |
| 0.2 | Magnesium chloride (MgCl 2 )(anhyd.) |
| 49.7 | 0.5231 |
| L-Asparagine | 26.6 |
| 0.2 | Magnesium sulfate(MgSO 4 )(anhyd.) |
| 192 | 1.600 |
| L-Cysteine hydrochloride hydrate | 70 |
| 0.3977 | Potassium Chloride (KCl) |
| 285 | 3.800 |
| L-Glutamic acid | 29 |
| 0.1973 | Sodium bicarbonate(NaHCO 3 ) |
| 2500 | 29.76 |
| L-Glutamine | – |
| - | Sodium chloride (NaCl) |
| 7530 | 129.9 |
| L-Histidine hydrochloride monohydrate | 45.8 |
| 0.2181 | Sodium Phosphate dibasic (Na 2 HPO 4 ) (anhyd.) |
| 115.5 | 0.813 |
| L-Isoleucine | 7.88 |
| 0.06015 | Sodium Phosphate monobasic (NaH 2 PO 4 ) (anhyd.) |
| 59.0 | 0.492 |
| L-Leucine | 26.2 |
| 0.2 | Zinc Sulfate(ZnSO 4 ·7H 2 O) |
| 0.144 | 0.0005 |
| L-Lysinehydrochloride | 73 |
| 0.3989 | Other components |
| L-Methionine | 8.96 |
| 0.06013 | D-Glucose (Dextrose) |
| 1260 | 7 |
| L-Phenylalanine | 9.92 |
| 0.06012 | Hypoxanthine |
| 4 | 0.03053 |
| L-Proline | 69 |
| 0.6 | Lipoic acid |
| 0.21 | 0.001019 |
| L-Serine | 21 |
| 0.2 | Phenol Red |
| 3 | 0.007970 |
| L-Threonine | 23 |
| 0.1933 | Putrescine 2HCl |
| 0.32 | 0.001987 |
| L-Tryptophan | 4.1 |
| 0.02010 | Sodium pyruvate |
| 220 | 2.0 |
| L-Tyrosine disodium salt | 13.5 |
| 0.05153 | Thymidine |
| 0.7 | 0.002892 |
| L-Valine | 23 |
| 0.1966 | Biotin |
| 0.0073 | 0.0000299 |
| Choline chloride | 14 |
| 0.1 | D-Calcium pantothenate |
| 0.50 | 0.001048 |
| Folic Acid | 1.30 |
| 0.002948 | Niacinamide |
| 0.04 | 0.000295 |
| Pyridoxine hydrochloride | 0.06 |
| 0.000291 | Riboflavin |
| 0.04 | 0.000098 |
| Thiamine hydrochloride | 0.30 |
| 0.000890 | Vitamin B12 |
| 1.40 | 0.001033 |
| i-Inositol | 18 |
| 0.1 |
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