Anti-S100 beta Rabbit pAb servicebio GB11359-100
$50.00
In stock
SKU
GB11359-100
Product Information
| Protein full name | Protein S100-B |
| Synonyms | NEF, S-100 protein beta chain, S-100 protein subunit beta, S100, S100 Beta, S100 calcium binding protein B, S100B, S100beta, S100-B, S100β |
| Uniprot ID | P50114 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse S100 beta |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Nucleus, Cytoplasm, Secreted |
Applications
| Applications: IHC/IF | Species: Mouse, Rat |
| Dilution: 1: 500-1: 4000 | Positive Tissue: brain |
Background
S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells.This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.
Images
IHC analysis of S100 beta(GB11359). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of S100 beta(GB11359). Sample: Rat brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IF analysis of S100 beta(GB11359)red. Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Reviews
Write Your Own Review