DMEM, High Glucose, GlutaPlus, 1.5 g/L Sodium Bicarbonate servicebio G4524-500ML
$50.00
In stock
SKU
G4524-500ML
Product Information
| Product Name | Cat. No. |
| Spec. | DMEM, high glucose, GlutaPlus, 1.5g/L sodium bicarbonate |
| G4524-500ML | 500 mL |
Product Description
Dulbecco's Modified Eagle Medium (DMEM), is a modified medium based on MEM, is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVEC and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7 and PC-12.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type. This product is a modified DMEM high glucose medium according to ATCC 30-2002. It contains 1.5 g/L sodium bicarbonate and sodium pyruvate.
This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 25 mM D-glucose, 1.5 g/L sodium bicarbonate, 4.0 mM L-alanyl-L-glutamine, 1.0 mM sodium pyruvate and phenol red indicator, no HEPES buffer system.
L-alanyl-L-glutamine, a cell culture additive, replaces medium molar L-glutamine in cell culture medium. The substance is very stable in aqueous solution and does not degrade spontaneously, it is slowly degraded by a peptidase secreted by the cells to release glutamine, which is then absorbed and used by the cells.
Storage and Shipping Conditions
Ship at room temperature; Store at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, ,do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves or protective clothing.
For Research Use Only!
G4524 DMEM (High Glucose) Medium Formula
Vitamins
| Components | Concentration |
| Components | Concentration |
| mg/L | mM |
| mg/L | mM |
| Amino acids | Inorganic salts |
| Glycine | 30 |
| 0.4 | Calcium Chloride (CaCl2) (anhyd.) |
| 200 | 1.80 |
| L-Arginine hydrochloride | 84 |
| 0.4 | Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) |
| 0.1 | 0.0002 |
| L-Cystine 2HCl | 62.6 |
| 0.2 | Magnesium Sulfate (MgSO4) (anhyd.) |
| 97.67 | 0.80 |
| L-Histidine hydrochloride-H2O | 42 |
| 0.2 | Potassium Chloride (KCl) |
| 400 | 5.36 |
| L-Isoleucine | 105 |
| 0.8 | Sodium Bicarbonate (NaHCO3) |
| 1500 | 17.85 |
| L-Leucine | 105 |
| 0.8 | Sodium Chloride (NaCl) |
| 6400 | 109.50 |
| L-Lysine hydrochloride | 146 |
| 0.8 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) |
| 108.7 | 0.906 |
| L-Methionine | 30 |
| 0.2 | Other components |
| L-Phenylalanine | 66 |
| 0.4 | D-Glucose (Dextrose) |
| 4500 | 24.98 |
| L-Serine | 42 |
| 0.4 | Sodium pyruvate |
| 110 | 1.0 |
| L-Threonine | 95 |
| 0.8 | HEPES |
| - | - |
| L-Tryptophan | 16 |
| 0.08 | Phenol Red |
| 14.95 | 0.0422 |
| L-Tyrosine disodium salt dihydrate dihydrate | 103.79 |
| 0.4 | L-Valine |
| 94 | 0.8 |
| L-Alanyl-L-Glutamine | 869 |
| 4.0 | Choline chloride |
| 4.0 | 0.0286 |
| D-Calcium pantothenate | 4.0 |
| 0.0084 | Folic Acid |
| 4.0 | 0.0091 |
| Niacinamide | 4.0 |
| 0.0328 | Pyridoxine hydrochloride |
| 4.0 | 0.0195 |
| Riboflavin | 0.4 |
| 0.0011 | Thiamine hydrochloride |
| 4.0 | 0.0119 |
| Meso-Inositol | 7.2 |
| 0.0389 |
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