MEM, GlutaPlus, no HEPES servicebio G4557-500ML
$50.00
In stock
SKU
G4557-500ML
Product Information
| Product Name: MEM, GlutaPlus, no HEPES | Cat. No.: G 4557-500ML |
| Spec.: 500 mL |
Product Description
MEM (Minimum Essential Medium), also known as minimum basic medium, low limit Eagle medium, is one of the most commonly used basic medium in animal cell culture. MEM contains only 12 amino acids, 8 vitamins and basic inorganic salts, with a simple nutrient composition, is mainly used for the culture of walled cells, but can also be used for other types of cell culture after the formulation is revised.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.
This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 5.5 mM D-glucose, 2.0 mM L-Alanyl-L-Glutamine and phenol red indicator, without sodium pyruvate, NEAA and HEPES.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves or protective clothing.
For Research Use Only!
G4557 MEM Formula
Vitamins
| Components | Concentration |
| Components | Concentration |
| mg/L | mM |
| mg/L | mM |
| Amino acids | Inorganic salts |
| L- A rginine hydrochloride | 126.0 |
| 0.5972 | Calcium Chloride (Ca Cl 2 ) (anhyd.) |
| 200 | 1.802 |
| L- C ystine 2HCl | 31.0 |
| 0.09904 | Magnesium Sulfate heptahydrate (MgSO 4 ·7H 2 O ) |
| 200 | 0.813 |
| L- A lanyl- L - G lutamine | 434.4 |
| 2.0 | Potassium Chloride (KCl) |
| 400 | 5.333 |
| L-Histidine hydrochloride-H 2 O | 42.0 |
| 0.20 | Sodium Bicarbonate (NaHCO 3 ) |
| 2200 | 26.19 |
| L-Isoleucine | 52.0 |
| 0.3969 | Sodium Chloride (NaCl) |
| 6350 | 109.5 |
| L-Leucine | 52.0 |
| 0.3969 | Sodium Phosphate monobasic (NaH 2 PO 4 ) (anhyd.) |
| 122 | 1.014 |
| L-Lysine hydrochloride | 73.0 |
| 0.3989 | Other components |
| L-Methionine | 15.0 |
| 0.1007 | D-Glucose (Dextrose) |
| 1000 | 5.556 |
| L-Phenylalanine | 32.0 |
| 0.1939 | Phenol Red |
| 9.4 | 0.02657 |
| L-Threonine | 48.0 |
| 0.4034 | HEPES |
| - | - |
| L- T ryptophan | 10.0 |
| 0.04902 | L-Tyrosine Disodium Salt Dihydrate |
| 52.0 | 0.1992 |
| L-Valine | 46.0 |
| 0.3932 | Glycine |
| - | - |
| L-A lanine | - |
| - | L-Asparagine mono hydrate |
| - | - |
| L-Aspartic Acid | - |
| - | L- G lutamic A cid |
| - | - |
| L-Proline | - |
| - | L-Serine |
| - | - |
| Choline chloride | 1.0 |
| 0. 007143 | D-Calcium pantothenate |
| 1.0 | 0.00 2096 |
| Folic Acid | 1.0 |
| 0.0022 68 | Niacinamide |
| 1.0 | 0.008 197 |
| Pyridoxine hydrochloride | 1.0 |
| 0.004 902 | Riboflavin |
| 0. 10 | 0.000 2660 |
| Thiamine hydrochloride | 1.0 |
| 0.0029 67 | Meso -Inositol |
| 2.0 | 0. 01111 |
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