Anti-Smad4 Rabbit pAb servicebio GB11174-100
$50.00
In stock
SKU
GB11174-100
Product Information
| Protein full name | Mothers against decapentaplegic homolog 4 |
| Synonyms | DPC4, JIP, MADH4, MYHRS, SMAD family member 4 |
| Uniprot ID | P97471 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Smad4 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 60 kDa / 70 kDa |
| Purity | Affinity purification |
Applications
| Applications: WB | Species: Human, Mouse, Rat |
| Dilution: 1: 500-1: 1000 | Positive Tissue: lung, stomach |
Background
Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta.
Images
WB analysis of Smad4(GB11174). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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