Glasgow's MEM (GMEM) servicebio G4558-500ML
$50.00
In stock
SKU
G4558-500ML
Product Information
| Product Name | Cat. No. |
| Spec. | Glasgow’s MEM (GMEM) |
| G4558-500ML | 500 mL |
Product Description
Glasgow's MEM (GMEM), originally developed by Ian McPherson and Michael Stoker for use with renal cell lines such as BGK-21, is a modified form of Eagle's minimum essential medium. It is used to study the genetic factors that influence the ability of cells. This product, Glasgow's MEM, contains amino acids and vitamins at twice the concentration of the original base Eagle medium and contains no proteins, lipids or growth factors. Therefore, before use, add a supplement of 10% tryptone phosphate broth as needed. Glasgow MEM uses a sodium bicarbonate buffer system (2.75 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This product is filtered and sterilized by 0.1 μm membrane, pH 6.7-7.1, containing 25 mM D-glucose, with 2.0 mM L-alanyl-L-glutamine added, no phenol red indicator, no sodium pyruvate, no HEPES buffer system, and no tryptone phosphate broth.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves or protective clothing.
For Research Use Only!
G4558 Glasgow’s MEM (GMEM)
Vitamins
| Components | Concentration |
| Components | Concentration |
| mg/L | mM |
| mg/L | mM |
| Amino acids | Inorganic salts |
| L-Arginine hydrochloride | 42 |
| 0.1990 | Calcium Chloride (CaCl2) (anhyd.) |
| 200 | 1.8018 |
| L-Cystine 2HCl | 31 |
| 0.0990 | Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) |
| 0.1 | 2.48E-04 |
| L-Glutamine | 292 |
| 2 | Magnesium Sulfate (MgSO4) (anhyd.) |
| 97.67 | 0.8139 |
| L-Histidine hydrochloride-H2O | 21 |
| 0.1 | Potassium Chloride (KCl) |
| 400 | 5.333 |
| L-Isoleucine | 52 |
| 0.3969 | Sodium Bicarbonate (NaHCO3) |
| 2750 | 32.738 |
| L-Leucine | 52 |
| 0.3969 | Sodium Chloride (NaCl) |
| 6400 | 110.34 |
| L-Lysine hydrochloride | 73 |
| 0.3989 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) |
| 108 | 0.8985 |
| L-Methionine | 15 |
| 0.1006 | Other components |
| L-Phenylalanine | 33 |
| 0.2 | D-Glucose (Dextrose) |
| 4500 | 25 |
| L-Threonine | 47.6 |
| 0.3999 | Phenol Red |
| 15 | 0.039 |
| L-Tryptophan | 8 |
| 0.0392 | L-Tyrosine disodium salt dihydrate dihydrate |
| 52 | 0.1992 |
| L-Valine | 46.8 |
| 0.4 | Choline chloride |
| 2.0 | 0.0142 |
| D-Calcium pantothenate | 2.0 |
| 0.0041 | Folic Acid |
| 2.0 | 0.0045 |
| Niacinamide | 2.0 |
| 0.0163 | Pyridoxine hydrochloride |
| 2.0 | 0.0098 |
| Riboflavin | 0.2 |
| 5.32E-04 | Thiamine hydrochloride |
| 2.0 | 0.0059 |
| Meso-Inositol | 3.6 |
| 0.02 |
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