DMEM, High Glucose, no L-Glutamine, no Calcium servicebio G4519-500ML
$50.00
In stock
SKU
G4519-500ML
Product Information
| Product Name | Cat. No. |
| Spec. | DMEM, high hlucose, no L-Glutamine, no calcium |
| G4519-500ML | 500 mL |
Product Description
Dulbecco's Modified Eagle Medium (DMEM), is a modified medium based on MEM, is now widely used in various cell cultures. DMEM High Glucose Medium is commonly used for fast-growing cells with low adhesion. It can be used for the culture of many kinds of mammalian primary cells, such as primary fibroblasts, neuronal cells, glial cells, HUVEC, smooth muscle cells, etc. It can also be used for the culture of some cell lines, such as Hela, 293, Cos-7 and PC-12.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients required for cell culture, does not contain protein or growth factors, need to be added according to the type of cells 5-10% serum or serum-free additives to use.
This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, containing 25 mM D-glucose, with phenol red indicator, calcium ion, L-glutamine, sodium pyruvate have not been added, and does not contain HEPES buffer system..
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. Avoid freezing the culture medium. Freezing may cause precipitation and precipitation of nutrients, which may not be completely dissolved after rewarming, affecting the cell culture effect. In addition, when the medium is stored at 2-8℃, do not store it completely close to the inner wall of the refrigerator to prevent the medium from freezing due to localized low temperature.
3. For your safety and health, please wear safety glasses, gloves or protective clothing.
For Research Use Only!
G4519 DMEM (High Glucose) Medium Formula
Vitamins
| Components | Concentration |
| Components | Concentration |
| mg/L | mM |
| mg/L | mM |
| Amino acids | Inorganic salts |
| Glycine | 30 |
| 0.4 | Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) |
| 0.1 | 0.0002 |
| L-Arginine hydrochloride | 84 |
| 0.4 | Magnesium Sulfate (MgSO4) (anhyd.) |
| 97.67 | 0.80 |
| L-Cystine 2HCl | 62.6 |
| 0.2 | Potassium Chloride (KCl) |
| 400 | 5.36 |
| L-Histidine hydrochloride-H2O | 42 |
| 0.2 | Sodium Bicarbonate (NaHCO3) |
| 3700 | 44.04 |
| L-Isoleucine | 105 |
| 0.8 | Sodium Chloride (NaCl) |
| 6400 | 109.50 |
| L-Leucine | 105 |
| 0.8 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) |
| 108.7 | 0.906 |
| L-Lysine hydrochloride | 146 |
| 0.8 | Other components |
| L-Methionine | 30 |
| 0.2 | D-Glucose (Dextrose) |
| 4500 | 24.98 |
| L-Phenylalanine | 66 |
| 0.4 | Phenol Red |
| 14.95 | 0.0422 |
| L-Serine | 42 |
| 0.4 | L-Threonine |
| 95 | 0.8 |
| L-Tryptophan | 16 |
| 0.08 | L-Tyrosine disodium salt dihydrate dihydrate |
| 103.79 | 0.4 |
| L-Valine | 94 |
| 0.8 | Choline chloride |
| 4.0 | 0.0286 |
| D-Calcium pantothenate | 4.0 |
| 0.0084 | Folic Acid |
| 4.0 | 0.0091 |
| Niacinamide | 4.0 |
| 0.0328 | Pyridoxine hydrochloride |
| 4.0 | 0.0195 |
| Riboflavin | 0.4 |
| 0.0011 | Thiamine hydrochloride |
| 4.0 | 0.0119 |
| Meso-Inositol | 7.2 |
| 0.0389 |
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