RPMI-1640 Medium, no L-Glutamine, no Calcium servicebio G4537-500ML
$50.00
In stock
SKU
G4537-500ML
Product Information
| Product Name: RPMI-1640 medium, no L-Glutamine, no calcium | Cat. No.: G 4537-500ML |
| Spec.: 500 mL |
Product Description
RPMI-1640 is a cell culture medium developed by the Roswell Park Memorial Institute (RPMI). This medium was originally designed specifically for lymphocytes culture, now is widely used for various normal cell and cancer cell culture, especially for cell suspension culture, and is one of the most widely used medium.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add 5-10% serum or serum-free additives depending on the cell type.
This product is filtered and sterilized by 0.1 μm membrane, pH 7.0-7.4, containing 11.1 mM D-glucose, phenol red indicator, calcium nitrate has not been added, no L-glutamine, no sodium pyruvate, no HEPES buffer system.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves or protective clothing.
For Research Use Only!
G4537 RPMI-1640 Formula
Vitamins
| Components | Concentration |
| Components | Concentration |
| mg/L | mM |
| mg/L | mM |
| Amino acids | Inorganic salts |
| Glycine | 10 |
| 0.133 | Magnesium Sulfate (MgSO 4 ) (anhyd.) |
| 48.84 | 0.407 |
| L- A rginine hydrochloride | 241.86 |
| 1.149 | Potassium Chloride (KCl) |
| 400 | 5.33 |
| L-Asparagine hydrate | 56.82 |
| 0.379 | Sodium Bicarbonate (NaHCO 3 ) |
| 2000 | 23.8 |
| L-Aspartic Acid | 20 |
| 0.15 | Sodium Chloride (NaCl) |
| 6000 | 103.4 |
| L- C ystine dihydrochloride | 65.2 |
| 0.208 | Sodium Phosphate monobasic (NaH 2 PO 4 ) (anhyd.) |
| 800 | 5.63 |
| L- G lutamic A cid | 20 |
| 0.136 | Other components |
| L-Histidine HCl H 2 O | 20.22 |
| 0.097 | D-Glucose (Dextrose) |
| 2000 | 11.11 |
| L-Hydroxyproline | 20 |
| 0.153 | Glutathione (reduced) |
| 1.0 | 0.003 |
| L-Isoleucine | 50 |
| 0.382 | Phenol Red |
| 4.6 | 0.013 |
| L-Leucine | 50 |
| 0.382 | L-Lysine hydrochloride |
| 40 | 0.219 |
| L-Methionine | 15 |
| 0.101 | L-Phenylalanine |
| 15 | 0.091 |
| L-Proline | 20 |
| 0.174 | L-Serine |
| 30 | 0.286 |
| L-Threonine | 20 |
| 0.168 | L- T ryptophan |
| 5.0 | 0.025 |
| L-Tyrosine Disodium Salt Dihydrate | 28.83 |
| 0.111 | L-Valine |
| 20 | 0.171 |
| L-Glutamine | - |
| - | L-Alanyl-L-Glutamine |
| - | - |
| Biotin | 0.2 |
| 0.00082 | Choline chloride |
| 3.0 | 0.02143 |
| D-Calcium pantothenate | 0.25 |
| 0.00052 | Folic Acid |
| 1.0 | 0.00227 |
| Niacinamide | 1.0 |
| 0.00820 | Para-Aminobenzoic Acid |
| 1.0 | 0.00730 |
| Pyridoxine hydrochloride | 1.0 |
| 0.00485 | Riboflavin |
| 0.2 | 0.00053 |
| Thiamine hydrochloride | 1.0 |
| 0.00297 | Vitamin B12 |
| 0.005 | 0.00000369 |
| i-Inositol | 35 |
| 0.19444 |
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