DMEM/no glucose, GlutaPlus, Sodium Pyruvate, no HEPES servicebio G4583-500ML
$50.00
In stock
SKU
G4583-500ML
Product Information
| Product Name | Cat. No. |
| Spec. | DMEM/no glucose, GlutaPlus, Sodium Pyruvate, no HEPES |
| G4583-500ML | 500 mL |
Product Description/Introduction
Dulbecco's Modified Eagle Medium (DMEM), is a modified medium based on MEM, is a widely used basal medium for supporting the growth of many different mammalian cells.. The DMEM medium was originally designed as low-glucose containing D-Glucose 1000 mg/L, and later developed as high-glucose containing D-Glucose 4500 mg/L. DMEM medium without D-glucose can be adjusted to suit the needs of the study.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.
The major character of this DMEM is as follows:
l Without glucose, HEPES
l With 4.0 mM L-alanyl-L-glutamine, 1.0 mM sodium pyruvate, phenol red.
The complete formulation is available..
Storage and Shipping Conditions
Ship at room temperature; Store at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
DMEM, no Glucose Formula
Vitamins
| Components | Concentration |
| Components | Concentration |
| mg/L | mM |
| mg/L | mM |
| Amino acids | Inorganic salts |
| Glycine | 30 |
| 0.4 | Calcium Chloride (CaCl2) (anhyd.) |
| 200 | 1.80 |
| L-Arginine hydrochloride | 84 |
| 0.4 | Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) |
| 0.1 | 0.0002 |
| L-Cystine dihydrochloride | 62.6 |
| 0.2 | Magnesium Sulfate (MgSO4) (anhyd.) |
| 97.67 | 0.80 |
| L-Histidine hydrochloride-H2O | 42 |
| 0.2 | Potassium Chloride (KCl) |
| 400 | 5.36 |
| L-Isoleucine | 105 |
| 0.80 | Sodium Bicarbonate (NaHCO3) |
| 3700 | 44.04 |
| L-Leucine | 105 |
| 0.80 | Sodium Chloride (NaCl) |
| 4750 | 81.9 |
| L-Lysine hydrochloride | 146 |
| 0.8 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) |
| 108.7 | 0.906 |
| L-Methionine | 30 |
| 0.2 | Other components |
| L-Phenylalanine | 66 |
| 0.4 | D-Glucose (Dextrose) |
| - | - |
| L-Serine | 42 |
| 0.4 | Sodium pyruvate |
| - | - |
| L-Threonine | 95 |
| 0.8 | HEPES |
| - | - |
| L-Tryptophan | 16 |
| 0.08 | Phenol Red |
| 14.95 | 0.0422 |
| L-Tyrosine disodium salt dihydrate | 103.79 |
| 0.4 | L-Valine |
| 94 | 0.8 |
| L-Glutamine | - |
| - | L-Alanyl-L-Glutamine |
| – | – |
| Choline chloride | 4.0 |
| 0.0286 | D-Calcium pantothenate |
| 4.0 | 0.0084 |
| Folic Acid | 4.0 |
| 0.0091 | Niacinamide |
| 4.0 | 0.0328 |
| Pyridoxine hydrochloride | 4.0 |
| 0.0195 | Riboflavin |
| 0.4 | 0.0011 |
| Thiamine hydrochloride | 4.0 |
| 0.0119 | i-Inositol |
| 7.2 | 0.0389 |
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