Anti-SYT1 Rabbit pAb servicebio GB111130-100
$50.00
In stock
SKU
GB111130-100
Product Information
| Protein full name | Synaptotagmin-1 |
| Synonyms | Synaptotagmin I, SytI, p65, DKFZp781D2042, FLJ42519, SVP65, Syt2 |
| Uniprot ID | P46096 |
| Immunogen | Recombinant protein corresponding to Mouse SYT1 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 47 kDa / 60 kDa |
| Purity | Affinity purification |
| Subcellular location | Cell Membrane |
Applications
| Applications: WB | Species: Mouse, Rat |
| Dilution: 1: 500-1: 1000 | Positive Tissue: brain, cerebral cortex, hippocampus |
| Applications: IHC/IF | Species: Human |
| Dilution: 1: 1000-1: 4000 | Positive Tissue: lung cancer |
Background
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin's Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse.
Images
WB analysis of SYT1(GB111130). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of SYT1(GB111130). Sample: Human lung cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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