Eagle's MEM (EMEM), powder servicebio G4650P-10L
$50.00
In stock
SKU
G4650P-10L
Product Information
| Product Name | Cat. No. |
| Spec. | Eagle's MEM (EMEM), powder |
| G4650P-1L | 1 L |
| G4650P-10L | 10 L |
| G4650P-50L | 50 L |
Product Description
Eagle's MEM (EMEM), is a modified medium to contain Earle's Balanced Salt Solution, non-essential amino acids, 1500 mg/L sodium bicarbonate. It has applications for cell culture, such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, desmocyte, etc.
This product is provided as powder, contains 5.55 mM D-glucose, 2.0 mM L-alanyl-L-glutamine, 1.0 mM sodium pyruvate, with phenol red indicator, without HEPES. The powder forms of culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocols below for details).
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8℃, valid for 36 months.
General guidelines
1. Please use sterile solutions of 1 N NaOH or HCl prepared by membrane iltration using appropriate ilters.
2. Filter using a low protein binding ilter.
3. Keep the container with medium covered during stirring and iltration.
4. Do not over-mix. Stop stirring when the product is completely dissolved.
Procotols of liquid media preparation from powder
1. To a mixing container that is as close to the final volume as possible, add 950 mL of distilled water.
2. Add Eagle's MEM (EMEM), powder medium 9.88 g to room temperature (15℃to 30℃) water (recommed WFI for cell culture or distilled water) with gentle stirring. Do not heat the water.
3. Rinse the inside of package to remove all traces of powder.
4. Add sodium bicarbonate (1.5 g/L) to the medium.
5. Adjust the pH to 0.2-0.3 units below the desires final working pH by slowly adding, with stirring, 1 N NaOH or 1 N HCl. The pH may rise 0.1 to 0.3 units upon filtration.
6. Adjust the final volume to 1000 mL with distilled water.
7. Process the medium immediately into sterile containers by membrane filtration with a 0.2-μm filter using a positive pressure system.
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