Product Information
| Product Name | Cat.No. |
| Spec. | DPBS Buffer, Containing Calcium and Magnesium Ions |
| G4201-500ML | 500 mL |
Description
DPBS buffer, namely Dulbecco's phosphate-buffered Saline, is a kind of balanced salt solution widely used in biological and biochemical studies, which has a slightly lower Phosphate content than conventional PBS buffer. DPBS is mainly used in embryological research, and can be used as rinse solution, cryopreserved solution and culture medium for embryos and tissues. Glucose and sodium pyruvate can be added to DPBS as needed to maintain the basic nutrition of the culture.
This product contains calcium and magnesium ions and does not contain phenol red.
Ingredients: 2.68 mM KCl, 1.47 mM KH2PO4, 137 mM NaCl, 8.0 mM Na2HPO4, 0.9 mM CaCl2, 0.49 mM MgCl2, pH 7.0-7.2@25℃. The bacteria were removed by 0.1 μm filtration.
Storage and Handling Conditions
Transport at room temperature; store at 2-8°C, valid for 24 months.
Component
DPBS Buffer Component
| Component | G4200-500ML |
| G4201-500ML | mg/L |
| mM | mg/L |
| mM | KCl |
| 200 | 2.68 |
| 200 | 2.68 |
| KH2PO4 | 200 |
| 1.47 | 200 |
| 1.47 | NaCl |
| 8000 | 137 |
| 8000 | 137 |
| Na2HPO4•12H2O | 2886 |
| 8.0 | 2886 |
| 8.0 | CaCl2 (anhydrous) |
| - | - |
| 100 | 0.9 |
| MgCl2•6H2O | - |
| - | 100 |
| 0.49 |
Note:
1. The product is sterilized by 0.1μm filtration and can be used directly.
2. This product contains calcium and magnesium ions, please keep it at 2-8℃. If it is not stored in the required conditions, it will produce trace precipitation and cannot be dissolved, so it cannot be used further.
3. Please pay attention to aseptic operation when using this product to avoid contamination.
For Research Use Only!