Product Information
| Protein full name | Double-stranded RNA-specific adenosine deaminase |
| Synonyms | DRADA, RNA adenosine deaminase 1, Adar, Adar1, 136kDa double stranded RNA binding protein, AGS6, Adenosine deaminase acting on RNA 1 A, Interferon inducible protein 4, RNA adenosine deaminase 1 |
| Uniprot ID | Q99MU3 |
| Immunogen | Recombinant protein corresponding to Mouse ADAR1 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 130 kDa / 110 kDa |
| Purity | Affinity purification |
Applications
| Applications: WB | Species: Human |
| Dilution: 1: 500-1: 1000 | Positive Tissue: HEK293T, Jurkat, HepG2 |
Background
ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region. The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an interferon (IFN) inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters.
Images
WB analysis of ADAR1(GB111458). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).