SWE Recombinant Trypsin (10×)-EDTA, no phenol red servicebio G4054-100ML
$50.00
In stock
SKU
G4054-100ML
Product Information
| Product Name | Cat. No. |
| Spec. | SWE Recombinant Trypsin (10×)-EDTA, no phenol red |
| G4054 | 100 mL |
Product Description/Introduction
Swe Recombinant Trypsin is an animal origin-free recombinant enzyme, it cleaves peptide bonds on the C-terminal side of lysine and arginine but with greater specificity than native trypsin preparations due to the superior purity.Swe Recombinant Trypsin (10×)-EDTA, phenol red, is a 10×Swe Recombinant Trypsin formulated in DPBS/1 mM EDTA without phenol red. It can be diluted to 1×using a balanced salt solution containing 1.1mMEDTA under sterile conditions, or directly used for dissociation of strongly adherent cells.
Features of this product: Gentle on cells, Room-temperature stable, Easy to use, Animal Origin Free (AOF).
Storage and Shipping Conditions
Store at room temperature or 4℃ protect from light..4℃ is better for preservation.
Shelf life: 12 months.
Shipping conditions: Ambient.
Product Content
| Component | G4054 |
| SWE Recombinant Trypsin (10×)-EDTA, no phenol red | 100 mL |
| Manual | 1 pc |
Assay Protocol / Procedures
1. (Optional) Swe Recombinant Trypsin (10×), diluted to 1× with balanced salt solution containing 1.1 mM EDTA under sterile conditions before use, and can also be used directly without dilution for strongly adherent cells.
2. Remove the cell culture medium and wash the cells 1-2 times with sterile PBS or D-PBS free of Ca2+ and Mg2+.
3. Add appropriate volume (e.g., 5 mL in a 75 cm2 flask) of Swe Recombinant Trypsin to cover the cells. Incubate at room temperature or 37°C until cells have detached. Observe cell monolayer using an inverted microscope to ensure complete cell detachment from the surface of the flask.
4. Add 5 mL of pre-warmed complete medium to flask. Collect the cell suspension in a centrifuge tube.
5. Centrifuge it at 800-1200 rpm/min for 5-10 min, then discard supernatant and resuspend cell pellet with 2–5 mL of pre-warmed complete medium for passaging culture or subsequent experiments.
Note
1. This product is very stable at room temperature, but it is still recommended to store it at 4°C. Avoid room temperature above 30℃. Avoid repeated freezing and thawing, if you need to store at -20℃.
2. This product is sterile and qualified by mycoplasma, endotoxin and other tests. Please pay attention to aseptic operation during use.
3. For your health and safety, please wear a lab coat and disposable gloves during operation..
For Research Use Only!
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