Product Information
| Protein full name | Tetratricopeptide repeat protein 33 |
| Synonyms | TPR repeat protein 33, Ttc33, OSRF, Osmosis-responsive factor |
| Uniprot ID | Q9D6K7 |
| Immunogen | Recombinant protein corresponding to Mouse TTC33 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 29 kDa / 34 kDa |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm, Nucleus |
Applications
| Applications: WB | Species: Human, Mouse |
| Dilution: 1: 500-1: 1000 | Positive Tissue: brain, kidney |
| Applications: IHC/IF | Species: Mouse, Rat |
| Dilution: 1: 650-1: 1300 | Positive Tissue: corpus striatum, prefrontal lobe |
Background
TTC33 contains 3 TPR repeats. The function of the TTC33 protein remains unknown.
Images
WB analysis of TTC33(GB112360). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IF analysis of TTC33(GB112360)red. Sample: Mouse prefrontal lobe, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 650, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of TTC33(GB112360)red. Sample: Rat corpus striatum, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 650, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).