Anti-GTF2E2 Rabbit pAb servicebio GB112155-100
$50.00
In stock
SKU
GB112155-100
Product Information
| Protein full name | General transcription factor IIE subunit 2 |
| Synonyms | Transcription initiation factor IIE subunit beta, TFIIE-beta, Gtf2e2, FE, TF2E2 |
| Uniprot ID | Q9D902 |
| Immunogen | Recombinant protein corresponding to Mouse GTF2E2 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 33 kDa / 33 kDa |
| Purity | Affinity purification |
| Subcellular location | Nucleus |
Applications
| Applications: WB | Species: Human, Mouse, Rat |
| Dilution: 1: 1000-1: 3000 | Positive Tissue: heart, brain, testis |
| Applications: IHC/IF | Species: Mouse, Rat |
| Dilution: 1: 600-1: 6000 | Positive Tissue: heart, liver, testis |
Background
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Images
WB analysis of GTF2E2(GB112155). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of GTF2E2(GB112155). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of GTF2E2(GB112155). Sample: Mouse liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of GTF2E2(GB112155). Sample: Mouse testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of GTF2E2(GB112155). Sample: Rat liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of GTF2E2(GB112155). Sample: Rat testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IF analysis of GTF2E2(GB112155)red. Sample: Rat liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of GTF2E2(GB112155)red. Sample: Rat testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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