Anti-HLA E Rabbit pAb servicebio GB112510-100
$50.00
In stock
SKU
GB112510-100
Product Information
| Protein full name | HLA class I histocompatibility antigen, alpha chain E |
| Synonyms | MHC class I antigen E, sHLA-E, HLA-6.2, HLAE, HLA-E, QA1, Major histocompatibility complex class I E, Lymphocyte antigen |
| Uniprot ID | P13747 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Human HLA E |
| Isotype | IgG |
| Predicted MW./Observed MW. | 40 kDa / 43 kDa |
| Purity | Affinity purification |
Applications
| Applications: WB | Species: Human |
| Dilution: 1: 350-1: 700 | Positive Tissue: HUVEC |
Background
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
Images
WB analysis of HLA E(GB112510). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 350, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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