Anti-TBX10 Rabbit pAb servicebio GB112700-100

$50.00
In stock
SKU
GB112700-100

 

Product Information

Protein full nameT-box transcription factor TBX10
SynonymsT-box protein 10, MmTBX7, T-box protein 13, Tbx10, Tbx13, Tbx7
Uniprot IDQ810F8
ImmunogenRecombinant protein corresponding to Mouse TBX10
IsotypeIgG
Predicted MW./Observed MW.42 kDa / 42 kDa
PurityAffinity purification
Subcellular locationNucleus

Applications

Applications: WBSpecies: Mouse, Rat
Dilution: 1: 500-1: 1000Positive Tissue: brain, skeletal muscle
Applications: IHC/IFSpecies: Human, Mouse, Rat
Dilution: 1: 2300-1: 4600Positive Tissue: esophageal cancer, liver, skeletal muscle

Background

Probable transcriptional regulator involved in developmental processes.

Images

WB analysis of TBX10(GB112700). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

WB analysis of TBX10(GB112700). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of TBX10(GB112700). Sample: Human esophagus cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 4600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of TBX10(GB112700). Sample: Mouse liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of TBX10(GB112700). Sample: Mouse Skeletal muscle, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of TBX10(GB112700). Sample: Mouse muscle, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of TBX10(GB112700). Sample: Rat muscle, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

StorageStore at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

 

 

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