Product Information
| Protein full name | Alanine--tRNA ligase, mitochondrial |
| Synonyms | Alanyl-tRNA synthetase, AlaRS, Aars2, Aarsl, Gm89, Kiaa1270, MT ALARS, MTALARS |
| Uniprot ID | Q14CH7 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse AARS2 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 107 kDa / 107 kDa |
| Purity | Affinity purification |
Applications
| Applications: WB | Species: Human |
| Dilution: 1: 300-1: 500 | Positive Tissue: K562 |
Background
AARS2(Alanine--tRNA ligase, mitochondrial) is also named as KIAA1270, AARSL, bA444E17.1, AlaRS, AARSL, COXPD8, MTALARS, MT-ALARS and belongs to the class-II aminoacyl-tRNA synthetase family. It catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). It also edits incorrectly charged tRNA(Ala) via its editing domain. The full length 107 kDa protein has a transit peptide with 23 amino acids.
Images
WB analysis of AARS2(GB113078). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).