Anti-PSMA3 Rabbit pAb servicebio GB113281-100
$50.00
In stock
SKU
GB113281-100
Product Information
| Protein full name | Proteasome subunit alpha type-3 |
| Synonyms | 20S proteasome, HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3 |
| Uniprot ID | O70435 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse PSMA3 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 28 kDa / 28 kDa |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm, Nucleus |
Applications
| Applications: WB | Species: Human, Mouse, Rat |
| Dilution: 1: 300-1: 600 | Positive Tissue: liver, spleen |
| Applications: IHC/IF | Species: Mouse, Rat |
| Dilution: 1: 1000-1: 2000 | Positive Tissue: spleen |
Background
PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.
Images
WB analysis of PSMA3(GB113281). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of PSMA3(GB113281). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of PSMA3(GB113281). Sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of PSMA3(GB113281). Sample: Rat spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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