Product Information
| Protein full name | Heat shock 70 kDa protein 1-like |
| Synonyms | Heat shock 70 kDa protein 1L, HSP70 1L, HSP70 HOM, HSP70T, HSPA1L, hum70t, Hsc70t, Spermatid-specific heat shock protein 70, Heat shock 70 kDa-like protein 1 |
| Uniprot ID | P16627 |
| Immunogen | Recombinant protein corresponding to Mouse HSPA1L |
| Isotype | IgG |
| Predicted MW./Observed MW. | 71 kDa / 71 kDa |
| Purity | Affinity purification |
| Subcellular location | Cell membrane, Nucleus, Cytoplasm, Mitochondrion |
Applications
| Applications: WB | Species: Human, Mouse, Rat |
| Dilution: 1: 1000-1: 2000 | Positive Tissue: placenta |
| Applications: IHC/IF | Species: Rat |
| Dilution: 1: 1400-1: 2800 | Positive Tissue: testis |
Background
HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognized the endogenous HSPA1L protein in multiple cell lines.
Images
WB analysis of HSPA1L(GB113554). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of HSPA1L(GB113554). Sample: Rat testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1400, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).