Anti-DNA polymerase eta Rabbit pAb servicebio GB113678-100
$50.00
In stock
SKU
GB113678-100
Product Information
| Protein full name | DNA polymerase eta |
| Synonyms | POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP-V, XPV, FLJ16395, FLJ21978, Xeroderma pigmentosum variant type protein homolog |
| Uniprot ID | Q9JJN0 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse DNA polymerase eta |
| Isotype | IgG |
| Predicted MW./Observed MW. | 76 kDa / 46 kDa |
| Purity | Affinity purification |
Applications
| Applications: WB | Species: Human, Mouse, Rat |
| Dilution: 1: 2000-1: 4000 | Positive Tissue: liver, kidney |
Background
This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination.
Images
WB analysis of DNA polymerase eta(GB113678). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 4000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of DNA polymerase eta(GB113678). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 4000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of DNA polymerase eta(GB113678). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 4000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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